Nagaie Maya, Nishiura Aki, Honda Yoshitomo, Fujiwara Shin-Ichi, Matsumoto Naoyuki
Department of Orthodontics, Graduate School of Dentistry, Osaka Dental University, 8-1, Kuzuha Hanazonocho, Hirakata, Osaka 573-1121, Japan.
Institute of Dental Research, Osaka Dental University, 8-1, Kuzuha Hanazonocho, Hirakata, Osaka 573-1121, Japan.
Int J Mol Sci. 2014 Oct 15;15(10):18610-22. doi: 10.3390/ijms151018610.
Tobacco smoke is a complex mixture of numerous components. Nevertheless, most experiments have examined the effects of individual chemicals in tobacco smoke. The comprehensive effects of components on tooth movement and bone resorption remain unexplored. Here, we have shown that a comprehensive mixture of tobacco smoke components (TSCs) attenuated bone resorption through osteoclastogenesis inhibition, thereby retarding experimental tooth movement in a rat model. An elastic power chain (PC) inserted between the first and second maxillary molars robustly yielded experimental tooth movement within 10 days. TSC administration effectively retarded tooth movement since day 4. Histological evaluation disclosed that tooth movement induced bone resorption at two sites: in the bone marrow and the peripheral bone near the root. TSC administration significantly reduced the number of tartrate-resistant acid phosphatase (TRAP)-positive osteoclastic cells in the bone marrow cavity of the PC-treated dentition. An in vitro study indicated that the inhibitory effects of TSCs on osteoclastogenesis seemed directed more toward preosteoclasts than osteoblasts. These results indicate that the comprehensive mixture of TSCs might be a useful tool for detailed verification of the adverse effects of tobacco smoke, possibly contributing to the development of reliable treatments in various fields associated with bone resorption.
烟草烟雾是多种成分的复杂混合物。然而，大多数实验都研究了烟草烟雾中单一化学物质的作用。其成分对牙齿移动和骨吸收的综合影响仍未得到探索。在此，我们表明，烟草烟雾成分（TSCs）的综合混合物通过抑制破骨细胞生成来减弱骨吸收，从而在大鼠模型中延缓实验性牙齿移动。在上颌第一和第二磨牙之间插入弹性动力链（PC）能在10天内有力地实现实验性牙齿移动。自第4天起，给予TSC能有效延缓牙齿移动。组织学评估显示，牙齿移动在两个部位诱导了骨吸收：骨髓和牙根附近的外周骨。给予TSC显著减少了PC处理牙列骨髓腔中抗酒石酸酸性磷酸酶（TRAP）阳性破骨细胞的数量。一项体外研究表明，TSCs对破骨细胞生成的抑制作用似乎更多地针对破骨前体细胞而非成骨细胞。这些结果表明，TSCs的综合混合物可能是详细验证烟草烟雾不良影响的有用工具，可能有助于在与骨吸收相关的各个领域开发可靠的治疗方法。
