Hamnvik Ole-Petter Riksfjord, Thakkar Bindiya, Chamberland John, Aronis Konstantinos, Schneider Benjamin, Mantzoros Christos S
Division of Endocrinology, Diabetes and Hypertension, Brigham and Women's Hospital, Harvard Medical School, Boston, MA.
Section of Endocrinology, Veteran's Affairs Boston Healthcare System, Harvard Medical School, Boston, MA.
Int J Obes (Lond). 2015 Feb;39(2):260-264. doi: 10.1038/ijo.2014.106. Epub 2014 Jun 20.
To study the day-night variation of omentin-1 levels and assess whether leptin and/or short- and long-term energy deprivation alter circulating omentin-1 levels via cytokines.
Omentin-1 levels were measured hourly in serum samples from six healthy men to evaluate for day-night variation. To study effects of acute energy deprivation and of leptin administration, eight healthy subjects were studied in the fasting state for 72 h with administration of either placebo or metreleptin (recombinant human leptin) in physiologic replacement doses. We evaluated the effect of leptin in pharmacologic doses on serum omentin-1 and cytokine levels, as well as on omentin-1 levels in ex vivo omental adipose tissue, in 15 healthy volunteers. To study the effect of chronic energy deprivation and weight loss on omentin-1 levels, we followed 18 obese subjects for 12 months who underwent bariatric surgery.
There is no day-night variation in omentin-1 levels. Short-term and chronic energy deprivation, as well as ex vivo leptin administration and physiologic replacement doses of leptin, do not alter omentin-1 levels; pharmacologic doses of metreleptin reduce omentin-1 levels, whereas levels of tumor necrosis factor-α receptor II and interleukin-6 tend to increase.
Omentin-1 levels are reduced by pharmacologic doses of metreleptin independent of effects on cytokine levels.
研究网膜素-1水平的昼夜变化，并评估瘦素和/或短期及长期能量剥夺是否通过细胞因子改变循环中的网膜素-1水平。
每小时测量6名健康男性血清样本中的网膜素-1水平，以评估昼夜变化。为研究急性能量剥夺和瘦素给药的影响，8名健康受试者在禁食状态下接受72小时研究，给予安慰剂或生理替代剂量的米泊明（重组人瘦素）。我们评估了药理剂量的瘦素对15名健康志愿者血清网膜素-1和细胞因子水平以及离体网膜脂肪组织中网膜素-1水平的影响。为研究慢性能量剥夺和体重减轻对网膜素-1水平的影响，我们对18名接受减肥手术的肥胖受试者进行了12个月的随访。
网膜素-1水平无昼夜变化。短期和慢性能量剥夺，以及离体给予瘦素和生理替代剂量的瘦素，均不会改变网膜素-1水平；药理剂量的米泊明可降低网膜素-1水平，而肿瘤坏死因子-α受体II和白细胞介素-6水平则趋于升高。
药理剂量的米泊明可降低网膜素-1水平，且与对细胞因子水平的影响无关。
