Section of Endocrinology, Boston VA Healthcare System, Harvard Medical School, Boston, Massachusetts 02130, USA.
Eur J Endocrinol. 2013 Sep 13;169(4):453-62. doi: 10.1530/EJE-13-0098. Print 2013 Oct.
Chemerin is an adipocyte-secreted hormone and has recently been associated with obesity and the metabolic syndrome. Although studies in rodents have outlined the aspects of chemerin's function and expression, its physiology and expression patterns are still to be elucidated in humans.
To evaluate for any day/night variation in chemerin secretion, we analyzed hourly serum samples from six females in the fed state. To examine whether energy deprivation affects chemerin levels, and whether this could be mediated through leptin, we analyzed samples from the same subjects in the fasting state while administering either placebo or leptin. To evaluate for any potential dose-effect relationship between leptin and chemerin, we administered increasing metreleptin doses to five females. A tissue array was used to study the expression of chemerin in different human tissues. Ex vivo treatment of human fat explants from three subjects with leptin was carried out to evaluate for any direct effect of leptin on adipocyte chemerin secretion.
Chemerin does not display a day/night variation, while acute energy deprivation resulted in a significant drop in circulating chemerin levels by ∼42%. The latter was unaltered by metreleptin administration, and leptin administration did not affect the secretion of chemerin by human adipose tissue studied ex vivo. Chemerin was expressed primarily in the pancreas and liver. Chemerin receptor showed increased expression in the lymph nodes and the spleen.
We outline for the first time chemerin expression and physiology in humans, which are different from those in mice.
趋化素是一种脂肪细胞分泌的激素，最近与肥胖和代谢综合征有关。虽然啮齿动物的研究概述了趋化素功能和表达的各个方面，但它在人类中的生理学和表达模式仍有待阐明。
为了评估趋化素分泌是否存在昼夜变化，我们分析了 6 名女性进食状态下每小时的血清样本。为了研究能量剥夺是否会影响趋化素水平，以及这种影响是否可以通过瘦素介导，我们分析了相同受试者在禁食状态下给予安慰剂或瘦素时的样本。为了评估瘦素和趋化素之间是否存在潜在的剂量-效应关系，我们给 5 名女性给予递增的 metreleptin 剂量。使用组织阵列研究了趋化素在不同人体组织中的表达。对来自 3 名受试者的人脂肪外植体进行了瘦素的离体处理，以评估瘦素对脂肪细胞趋化素分泌的直接影响。
趋化素没有昼夜变化，而急性能量剥夺导致循环趋化素水平显著下降约 42%。后者不受 metreleptin 给药的影响，而瘦素给药并未影响离体研究的人脂肪组织中趋化素的分泌。趋化素主要在胰腺和肝脏中表达。趋化素受体在淋巴结和脾脏中的表达增加。
我们首次概述了人类趋化素的表达和生理学，这与小鼠的不同。
