Tandara M, Bajić A, Tandara L, Bilić-Zulle L, Šunj M, Kozina V, Goluža T, Jukić M
Department of Human Reproduction, Clinic for Women's Diseases and Obstetrics of University Hospital Center Split, Split, Croatia.
Andrology. 2014 Sep;2(5):678-86. doi: 10.1111/j.2047-2927.2014.00234.x. Epub 2014 Jun 19.
Sperm DNA integrity is a sperm functional parameter of male fertility evaluation. Two parameters of sperm DNA integrity were observed: DNA damage expressed as DNA fragmentation index (DFI) and percentage of the DNA undamaged spermatozoa expressed as big halo. Halosperm test was used for sperm DNA integrity determination. The aim of this study was to evaluate which DNA integrity parameter is better as an embryo quality and pregnancy prognostic parameter after the conventional IVF. We evaluated two embryo groups (positive and negative group) according to the 3rd day cumulative embryo score. Big halo and DFI, as we expected, showed good correlation (r = -0.69; p < 0.001). Receiver operating characteristic (ROC) analyses show that DFI and big halo are significant (p < 0.001) as prognostic parameters of embryo quality. ROC curves comparison of DFI and big halo revealed the AUC value for big halo to be significantly higher (DFI AUC = 0.71 vs. big halo AUC = 0.83; p = 0.025) than for DFI. Big halo was found to be the only independent predictor of embryo quality. Sperm DNA integrity both parameters are good prognostic parameters of embryo quality after the conventional IVF where big halo seems to be better. ROC analyses show DFI and big halo as significant prognostic parameters for achieved pregnancy (AUC ± SE for DFI was 0.67 ± 0.06 and 0.75 ± 0.06 for big halo). To our knowledge, this is the first study demonstrating the correlation between sperm DNA undamaged rate expressed as big halo parameter and semen characteristics as well as the influence on fertilization rate, embryo quality and pregnancy in conventional IVF.
精子DNA完整性是男性生育力评估中的一个精子功能参数。观察了两个精子DNA完整性参数：以DNA碎片化指数（DFI）表示的DNA损伤以及以大晕环表示的未受损精子DNA的百分比。采用晕环精子试验来测定精子DNA完整性。本研究的目的是评估在常规体外受精后，哪个DNA完整性参数作为胚胎质量和妊娠预后参数更好。我们根据第3天的累积胚胎评分评估了两个胚胎组（阳性组和阴性组）。正如我们所预期的，大晕环和DFI显示出良好的相关性（r = -0.69；p < 0.001）。受试者工作特征（ROC）分析表明，DFI和大晕环作为胚胎质量的预后参数具有显著性（p < 0.001）。DFI和大晕环的ROC曲线比较显示，大晕环的AUC值显著高于DFI（DFI的AUC = 0.71，大晕环的AUC = 0.83；p = 0.025）。发现大晕环是胚胎质量的唯一独立预测指标。在常规体外受精后，精子DNA完整性的这两个参数都是胚胎质量的良好预后参数，其中大晕环似乎更好。ROC分析表明，DFI和大晕环是妊娠成功的显著预后参数（DFI的AUC ± SE为0.67 ± 0.06，大晕环为0.75 ± 0.06）。据我们所知，这是第一项证明以大晕环参数表示的未受损精子DNA率与精液特征之间的相关性以及对常规体外受精中受精率、胚胎质量和妊娠影响的研究。
