Shuai Peng, Liang Dan, Tang Sha, Zhang Zhoujia, Ye Chu-Yu, Su Yanyan, Xia Xinli, Yin Weilun
College of Biological Sciences and Technology, National Engineering Laboratory of Tree Breeding, Beijing Forestry University, mailbox 69, No. 35 Qinghua East Road, Haidian District, Beijing 100083, P.R. China.
College of Biological Sciences and Technology, National Engineering Laboratory of Tree Breeding, Beijing Forestry University, mailbox 69, No. 35 Qinghua East Road, Haidian District, Beijing 100083, P.R. China
J Exp Bot. 2014 Sep;65(17):4975-83. doi: 10.1093/jxb/eru256. Epub 2014 Jun 19.
Protein-coding genes are considered to be a dominant component of the eukaryotic transcriptome; however, many studies have shown that intergenic, non-coding transcripts also play an important role. Long intergenic non-coding RNAs (lincRNAs) were found to play a vital role in human and Arabidopsis. However, lincRNAs and their regulatory roles remain poorly characterized in woody plants, especially Populus trichocarpa (P. trichocarpa). A large set of Populus RNA-Seq data were examined with high sequencing depth under control and drought conditions and a total of 2542 lincRNA candidates were identified. In total, 51 lincRNAs and 20 lincRNAs were identified as putative targets and target mimics of known Populus miRNAs, respectively. A total of 504 lincRNAs were found to be drought responsive, eight of which were confirmed by RT-qPCR. These findings provide a comprehensive view of Populus lincRNAs, which will enable in-depth functional analysis.
蛋白质编码基因被认为是真核转录组的主要组成部分；然而，许多研究表明，基因间的非编码转录本也发挥着重要作用。长基因间非编码RNA（lincRNA）被发现在人类和拟南芥中起着至关重要的作用。然而，lincRNA及其调控作用在木本植物中，尤其是毛果杨（P. trichocarpa）中，仍未得到充分表征。在对照和干旱条件下，对大量毛果杨RNA-Seq数据进行了高测序深度检测，共鉴定出2542个lincRNA候选物。总共分别鉴定出51个lincRNA和20个lincRNA作为已知毛果杨miRNA的假定靶标和靶标模拟物。共发现504个lincRNA对干旱有响应，其中8个通过RT-qPCR得到证实。这些发现提供了毛果杨lincRNA的全面视图，这将有助于进行深入的功能分析。
