氯化锂-苯乙醇-莫西沙星与改良Vogel Johnson琼脂用于检测零售肉类、家禽和海鲜中李斯特菌属的比较。
Comparison of lithium chloride-phenylethanol-moxalactam and modified Vogel Johnson agars for detection of Listeria spp. in retail-level meats, poultry, and seafood.
作者信息
Buchanan R L, Stahl H G, Bencivengo M M, Del Corral F
机构信息
Eastern Regional Research Center, U.S. Department of Agriculture, Philadelphia, Pennsylvania 19118.
出版信息
Appl Environ Microbiol. 1989 Mar;55(3):599-603. doi: 10.1128/aem.55.3.599-603.1989.
Abstract
The effectiveness of Modified Vogel Johnson agar and lithium chloride-phenylethanol-moxalactam agar for detection of Listeria spp. in foods was compared by using the media to analyze retail-level meat, poultry, and seafood both by direct plating and in conjunction with a three-tube most-probable-number enrichment. The most-probable-number protocol detected Listeria species, including Listeria monocytogenes, in a substantial portion of the fresh meat and seafood samples. In most instances the Listeria levels were less than 2 CFU/g, which precluded detection by direct plating. Modified Vogel Johnson agar performed as well as did lithium chloride-phenylethanol-moxalactam agar and was considerably easier to use because of its ability to differentiate Listeria spp. from other microorganisms.
摘要
通过直接平板接种以及结合三管最大可能数富集法，使用改良的Vogel Johnson琼脂和氯化锂-苯乙醇-莫西酰胺琼脂对食品中的李斯特菌属进行检测，比较了这两种培养基在分析零售层面的肉类、禽类和海产品时的有效性。最大可能数方案在相当一部分鲜肉和海产品样本中检测到了李斯特菌属，包括产单核细胞李斯特菌。在大多数情况下，李斯特菌水平低于2 CFU/g，这使得通过直接平板接种无法检测到。改良的Vogel Johnson琼脂与氯化锂-苯乙醇-莫西酰胺琼脂表现相当，并且由于其能够将李斯特菌属与其他微生物区分开来，使用起来要容易得多。
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