Department of Chemistry and Biochemistry, University of California-Los Angeles, Los Angeles, CA, 90095, USA.
Adv Exp Med Biol. 2014;806:283-99. doi: 10.1007/978-3-319-06068-2_12.
Mass spectrometry imaging (MSI) of tissue samples is a promising analytical tool that has quickly become associated with biomedical and pharmacokinetic studies. It eliminates several labor-intensive protocols associated with more classical imaging techniques and provides accurate histological data at a rapid pace. Because mass spectrometry is used as the readout, MSI can be applied to almost any molecule, especially those that are biologically relevant. Many examples of its utility in the study of peptides and proteins have been reported; here we discuss its value in the mass range of small molecules. We explore its success and potential in the analysis of lipids, medicinals, and metal-based compounds by featuring representative studies from MSI laboratories around the globe.
组织样本的质谱成像（MSI）是一种很有前途的分析工具，它已迅速与生物医学和药代动力学研究相关联。它消除了与更经典的成像技术相关的几个劳动密集型方案，并以快速的速度提供准确的组织学数据。由于质谱用作读出，MSI 可以应用于几乎任何分子，特别是那些具有生物学相关性的分子。已经报道了其在肽和蛋白质研究中的许多应用实例；在这里，我们讨论了其在小分子质量范围内的价值。我们通过全球 MSI 实验室的代表性研究来探讨其在脂质、药物和基于金属的化合物分析中的成功和潜力。
