Rahman M, Noble W C, Cookson B
Department of Microbiology, Institute of Dermatology, United Medical School, London.
Epidemiol Infect. 1989 Apr;102(2):261-70. doi: 10.1017/s0950268800029939.
The spread of two strains of Staphylococcus aureus with high level resistance to mupirocin is described. The resistance proved to be easily transferred to other S. aureus strains by filter mating experiments and on the skin of mice. No plasmid band corresponding to the resistance could be demonstrated by agarose gel electrophoresis or by caesium chloride gradient centrifugation but cleavage of 'chromosomal' DNA from resistant recipients showed bright bands of DNA absent from sensitive controls.
本文描述了两株对莫匹罗星具有高度耐药性的金黄色葡萄球菌的传播情况。通过滤膜交配实验以及在小鼠皮肤上进行实验，证实这种耐药性很容易转移至其他金黄色葡萄球菌菌株。通过琼脂糖凝胶电泳或氯化铯梯度离心法，均未发现与该耐药性相对应的质粒条带，但是从耐药受体中提取的“染色体”DNA经酶切后，出现了敏感对照中没有的明亮DNA条带。
