Dewi Beti Ernawati, Naiggolan Leonard, Putri Dwi Hilda, Rachmayanti Novia, Albar Sarah, Indriastuti Nadia Tita, Sjamsuridzal Wellyzar, Sudiro T Mirawati
Southeast Asian J Trop Med Public Health. 2014 Jan;45(1):53-61.
Dengue hemorrhagic fever has become a worldwide health issue. Heterologous infection by different serotypes may lead to severe forms of dengue infection and even death. In a cohort study in Jakarta from 2009 to 2010 with inclusion criteria of adults with fever of less than 48 hours, 72% were confirmed dengue infection from a total of suspected 190 dengue patients. Using RT-PCR, 16 patients were infected with DENV-4 with or without co-infection with other serotype(s). Dengue fever (DF) patients (73%) were infected with DENV-4 alone, while mixed infections were present in more severe clinical manifestations of DHF grade I and II. The nucleotide sequences of envelope (E) protein gene of 3 isolates of DENV-4 showed that they belonged to genotype II. There were 50 nucleotide substitutions, but only 3 amino acid changes were found at positions 130, 233 and 455, present in E protein isolated from DHF grade II patient.
登革出血热已成为一个全球性的健康问题。不同血清型的异源感染可能导致严重的登革热感染甚至死亡。在2009年至2010年于雅加达进行的一项队列研究中，纳入标准为发热不到48小时的成年人，在总共190名疑似登革热患者中，72%被确诊为登革热感染。使用逆转录聚合酶链反应（RT-PCR），16名患者感染了登革病毒4型（DENV-4），有或无其他血清型的共同感染。登革热（DF）患者（73%）仅感染了DENV-4，而在登革出血热I级和II级更严重的临床表现中存在混合感染。3株DENV-4包膜（E）蛋白基因的核苷酸序列表明它们属于基因型II。有50个核苷酸替换，但在从登革出血热II级患者分离的E蛋白中，仅在第130、233和455位发现3个氨基酸变化。
