Pfaffenwimmer Thaddaeus, Reiter Wolfgang, Brach Thorsten, Nogellova Veronika, Papinski Daniel, Schuschnig Martina, Abert Christine, Ammerer Gustav, Martens Sascha, Kraft Claudine
Max F. Perutz Laboratories, University of Vienna, Vienna, Austria.
Max F. Perutz Laboratories, University of Vienna, Vienna, Austria
EMBO Rep. 2014 Aug;15(8):862-70. doi: 10.15252/embr.201438932. Epub 2014 Jun 26.
Autophagy is the major pathway for the delivery of cytoplasmic material to the vacuole or lysosome. Selective autophagy is mediated by cargo receptors, which link the cargo to the scaffold protein Atg11 and to Atg8 family proteins on the forming autophagosomal membrane. We show that the essential kinase Hrr25 activates the cargo receptor Atg19 by phosphorylation, which is required to link cargo to the Atg11 scaffold, allowing selective autophagy to proceed. We also find that the Atg34 cargo receptor is regulated in a similar manner, suggesting a conserved mechanism.
自噬是将细胞质物质运输到液泡或溶酶体的主要途径。选择性自噬由货物受体介导，货物受体将货物与支架蛋白Atg11以及正在形成的自噬体膜上的Atg8家族蛋白相连。我们发现，必需激酶Hrr25通过磷酸化激活货物受体Atg19，这是将货物与Atg11支架相连所必需的，从而使选择性自噬得以进行。我们还发现，Atg34货物受体也以类似方式受到调控，这表明存在一种保守机制。
