Departamento de Química Inorgánica, Facultad de Ciencias, Universidad de Málaga, Campus de Teatinos s/n, 29071, Málaga, Spain.
Nanoscale. 2014 Aug 7;6(15):9071-7. doi: 10.1039/c4nr01585a.
Highly photoluminescent carbon dots have been prepared in a one step procedure by hydrothermal treatment of formaldehyde at 180 °C. They show green fluorescence under UV light exposure and emission spectra are centered at 440 nm. Fluorescence lifetimes comprise between 0.7 and 2.70 ns, when the synthesis process lasted for 1-7 days. TEM images of nanoparticles showed a homogeneous size/shape distribution. When the thermal treatment process was carried out for a long time (30 days) formation of aggregates occurred. Carbon dots were further analyzed using (1)H and (13)C-NMR, Raman and FTIR spectroscopy techniques and XPS. Cell imaging of nanoparticles was carried out by using mouse MC3T3-E1 pre-osteoblasts as a model. The nanoparticles were selectively localized in the cytoplasm without further functionalization and could be realized by cellular phagocytosis, so that the fluorescence of these can be used for live cell imaging in vitro.
通过在 180°C 下将甲醛水热处理，一步法制备了具有高光致发光性的碳点。它们在紫外光照射下发出绿色荧光，发射光谱的中心位于 440nm 处。当合成过程持续 1-7 天时，荧光寿命在 0.7 到 2.70 ns 之间。当热解时间延长至 30 天时，纳米颗粒会发生团聚。通过 1H 和 13C-NMR、拉曼和傅里叶变换红外光谱以及 XPS 对碳点进一步分析。以小鼠 MC3T3-E1 前成骨细胞为模型进行纳米颗粒的细胞成像。纳米颗粒未经进一步功能化即可选择性定位于细胞质中，并可通过细胞吞噬作用来实现，因此这些颗粒的荧光可用于体外活细胞成像。
