Moon H, Hong M, Kim J K, Seon B, Na W, Park S J, An D J, Jeoung H Y, Kim D J, Kim J M, Kim S H, Webby R J, Webster R G, Kang B K, Song D
Research Unit,Green Cross Veterinary Products, Yongin, Gyeonggi,Republic of Korea.
Viral Infectious Disease Research Center,Korea Research Institute of Bioscience and Biotechnology,Daejeon,Republic of Korea.
Epidemiol Infect. 2015 Mar;143(4):772-80. doi: 10.1017/S0950268814001617. Epub 2014 Jun 30.
After an outbreak of pandemic influenza A/H1N1 (pH1N1) virus, we had previously reported the emergence of a recombinant canine influenza virus (CIV) between the pH1N1 virus and the classic H3N2 CIV. Our ongoing routine surveillance isolated another reassortant H3N2 CIV carrying the matrix gene of the pH1N1 virus from 2012. The infection dynamics of this H3N2 CIV variant (CIV/H3N2mv) were investigated in dogs and ferrets via experimental infection and transmission. The CIV/H3N2mv-infected dogs and ferrets produced typical symptoms of respiratory disease, virus shedding, seroconversion, and direct-contact transmissions. Although indirect exposure was not presented for ferrets, CIV/H3N2mv presented higher viral replication in MDCK cells and more efficient transmission was observed in ferrets compared to classic CIV H3N2. This study demonstrates the effect of reassortment of the M gene of pH1N1 in CIV H3N2.
在甲型H1N1大流行性流感病毒（pH1N1）爆发后，我们之前曾报道过一种在pH1N1病毒与经典H3N2犬流感病毒（CIV）之间出现的重组犬流感病毒。我们正在进行的常规监测从2012年的样本中分离出了另一种携带pH1N1病毒基质基因的重组H3N2 CIV。通过实验性感染和传播，在犬类和雪貂中对这种H3N2 CIV变体（CIV/H3N2mv）的感染动态进行了研究。感染CIV/H3N2mv的犬类和雪貂出现了呼吸道疾病的典型症状、病毒脱落、血清转化以及直接接触传播。虽然未对雪貂进行间接暴露实验，但与经典的CIV H3N2相比，CIV/H3N2mv在MDCK细胞中表现出更高的病毒复制水平，并且在雪貂中观察到了更有效的传播。这项研究证明了pH1N1的M基因重排在CIV H3N2中的作用。
