啮齿动物虹膜的体内光学相干断层扫描微血管造影
In vivo OCT microangiography of rodent iris.
作者信息
Choi Woo June, Zhi Zhongwei, Wang Ruikang K
出版信息
Opt Lett. 2014 Apr 15;39(8):2455-8. doi: 10.1364/OL.39.002455.
Abstract
We report on the functional optical coherence tomography (OCT) imaging of iris tissue morphology and microcirculation in living small animals. Anterior segments of healthy mouse and rat eyes are imaged with high-speed spectral domain OCT (SD-OCT) utilizing ultrahigh sensitive optical microangiography (UHS-OMAG) imaging protocol. 3D iris microvasculature is produced by the use of an algorithm that calculates absolute differences between the amplitudes of the OCT interframes. We demonstrate that the UHS-OMAG is capable of delineating iris microvascular beds in the mouse and rat with capillary-level resolution. Furthermore, the fast imaging speed enables dynamic imaging of iris micro-vascular response during drug-induced pupil dilation. We believe that this OCT angiographic approach has a great potential for in situ and in vivo monitoring of the microcirculation within iris tissue beds in rodent disease models that have microvascular involvement.
摘要
我们报告了在活体小动物中对虹膜组织形态和微循环进行功能性光学相干断层扫描（OCT）成像的情况。利用超高灵敏度光学微血管造影（UHS-OMAG）成像协议，通过高速光谱域OCT（SD-OCT）对健康小鼠和大鼠眼睛的前段进行成像。通过使用一种计算OCT帧间振幅绝对差值的算法生成三维虹膜微血管系统。我们证明，UHS-OMAG能够以毛细血管水平的分辨率描绘小鼠和大鼠的虹膜微血管床。此外，快速成像速度能够对药物诱导瞳孔扩张期间的虹膜微血管反应进行动态成像。我们认为，这种OCT血管造影方法在原位和体内监测有微血管受累的啮齿动物疾病模型中虹膜组织床内的微循环方面具有巨大潜力。
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