Biophotonics and Imaging Laboratory, Division of Biomedical Engineering, School of Medicine, Oregon Health and Science University, Portland, OR 97239, USA.
IEEE Trans Med Imaging. 2011 Feb;30(2):224-30. doi: 10.1109/TMI.2010.2072934. Epub 2010 Sep 2.
Studying the inner ear microvascular dynamics is extremely important to understand the cochlear function and to further advance the diagnosis, prevention, and treatment of many otologic disorders. However, there is currently no effective imaging tool available that is able to access the blood flow within the intact cochlea. In this paper, we report the use of an ultrahigh sensitive optical micro-angiography (UHS-OMAG) imaging system to image 3-D microvascular perfusion within the intact cochlea in living mice. The UHS-OMAG image system used in this study is based on spectral domain optical coherence tomography, which uses a broadband light source centered at 1300 nm with an imaging rate of 47[Formula: see text] 000 A-scans/s, capable of acquiring high-resolution B scans at 300 frames/s. The technique is sensitive enough to image very slow blood flow velocities, such as those found in capillary networks. The 3-D imaging acquisition time for a whole cochlea is ∼ 4.1 s. We demonstrate that volumetric reconstruction of microvascular flow obtained by UHS-OMAG provides a comprehensive perfusion map of several regions of the cochlea, including the otic capsule, the stria vascularis of the apical and middle turns and the radiating arterioles that emanate from the modiolus.
研究内耳微血管动力学对于理解耳蜗功能以及进一步推进许多耳科学疾病的诊断、预防和治疗至关重要。然而，目前尚无有效的成像工具能够获取完整耳蜗内的血流信息。本文报道了使用超高灵敏度光学微血管造影（UHS-OMAG）成像系统来对活体小鼠完整耳蜗内的三维微血管灌注进行成像。本研究中使用的 UHS-OMAG 成像系统基于光谱域光相干断层扫描技术，使用中心波长为 1300nm 的宽带光源，成像速率为 47000000A 扫描/秒，能够以 300 帧/秒的速度获取高分辨率 B 扫描。该技术的灵敏度足以对毛细血管网络中发现的非常缓慢的血流速度进行成像。整个耳蜗的三维成像采集时间约为 4.1 秒。我们证明，UHS-OMAG 获得的微血管流动容积重建提供了耳蜗几个区域的全面灌注图，包括耳囊、耳蜗顶回和中回的血管纹以及从中轴发出的辐射状动脉。
