Jahns Anika C, Alexeyev Oleg A
Department of Medical Biosciences/Pathology, Umeå University, Umeå, Sweden.
Exp Dermatol. 2014 Sep;23(9):687-9. doi: 10.1111/exd.12482.
Propionibacterium acnes is regarded as a common member of the human skin microbiota, often occurring in biofilms. Little is known about the size of bacterial biofilms in hair follicles as a few sections of biopsy tissue are routinely evaluated. Transversal sectioning provides a better opportunity for histological analyses of hair follicles which can be followed through the different morphological levels. Direct visualization of P. acnes biofilms in hundreds of consecutive sections allowed insight into the 3D distribution in human hair follicles as well as investigating the depth of biofilm distribution within hair follicles. Four distinct colonization patterns of P. acnes biofilms were revealed. Results have shown that an individual P. acnes biofilm can spread for 1900 μm in a terminal hair follicle. This information can be of help while designing potential antibiofilm treatment.
痤疮丙酸杆菌被认为是人类皮肤微生物群的常见成员，常出现在生物膜中。由于通常只对少数活检组织切片进行评估，因此对毛囊中细菌生物膜的大小了解甚少。横向切片为毛囊的组织学分析提供了更好的机会，可以在不同的形态学层面进行观察。通过对数百个连续切片中痤疮丙酸杆菌生物膜的直接可视化，能够深入了解其在人类毛囊中的三维分布，并研究生物膜在毛囊内的分布深度。研究揭示了痤疮丙酸杆菌生物膜的四种不同定植模式。结果表明，单个痤疮丙酸杆菌生物膜在终末毛囊中可扩散达1900μm。这些信息在设计潜在的抗生物膜治疗方案时可能会有所帮助。
