Lani Athina, Kourkoumelis Nikolaos, Baliouskas Gerasimos, Tzaphlidou Margaret
Department of Medical Physics, Medical School, University of Ioannina, Ioannina, 45110, Greece.
J Biol Phys. 2014 Sep;40(4):401-12. doi: 10.1007/s10867-014-9358-8. Epub 2014 Jul 9.
Fourier transform infrared spectroscopy is utilized to examine the effects of increased calcium, vitamin D, and combined calcium-vitamin D supplementation on osteoporotic rabbit bones with induced inflammation. The study includes different bone sites (femur, tibia, humerus, vertebral rib) in an effort to explore possible differences among the sites. We evaluate the following parameters: mineral-to-matrix ratio, carbonate content, and non-apatitic species (labile acid phosphate and labile carbonate) contribution to bone mineral. Results show that a relatively high dose of calcium or calcium with vitamin D supplementation increases the bone mineralization index significantly. On the other hand, vitamin D alone is not as effective in promoting mineralization even with high intake. Mature B-type apatite was detected for the group with calcium supplementation similar to that of aged bone. High vitamin D intake led to increased labile species concentration revealing bone formation. This is directly associated with the suppression of pro-inflammatory cytokines linked to induced inflammation. The latter is known to adversely alter bone metabolism, contributing to the aetiopathogenesis of osteoporosis. Thus, a high intake of vitamin D under inflammation-induced osteoporosis does not promote mineralization but suppresses bone resorption and restores metabolic balance.
傅里叶变换红外光谱法用于研究增加钙、维生素D以及联合补充钙和维生素D对患有炎症的骨质疏松兔骨的影响。该研究包括不同的骨部位（股骨、胫骨、肱骨、椎骨肋骨），以探索各部位之间可能存在的差异。我们评估以下参数：矿物质与基质的比例、碳酸盐含量以及非磷灰石种类（不稳定酸性磷酸盐和不稳定碳酸盐）对骨矿物质的贡献。结果表明，相对高剂量的钙或钙与维生素D联合补充可显著提高骨矿化指数。另一方面，即使高剂量摄入，单独补充维生素D在促进矿化方面效果不佳。补充钙的组检测到成熟的B型磷灰石，与老年骨相似。高剂量维生素D摄入导致不稳定种类浓度增加，表明有骨形成。这与抑制与炎症诱导相关的促炎细胞因子直接相关。后者已知会对骨代谢产生不利影响，导致骨质疏松症的发病机制。因此，在炎症诱导的骨质疏松症情况下，高剂量摄入维生素D不会促进矿化，而是抑制骨吸收并恢复代谢平衡。
