Jones M V, Herd T M, Christie H J
Unilever Research, Bedford, Great Britain.
Microbios. 1989;58(234):49-61.
Pseudomonas aeruginosa was able to grow in high levels of an amphoteric and a quaternary ammonium compound following repeated subculturing in increasing concentrations of the biocides. Resistance was acquired and lost gradually. Adaptation to both biocides resulted in cross resistance to biguanides but whereas quaternary adapted cells were resistant to a range of quaternary ammonium compounds, the amphoteric adapted organisms were not. Amphoteric adapted cells had increased hydrophobicity and exhibited ultrastructural modifications which suggested that the outer membrane might be involved in resistance. Both amphoteric and quaternary ammonium adapted organisms showed changes in their fatty acid profiles consistent with outer membrane modification but the changes were different in each case. The mechanisms involved in biocide resistance are discussed.
铜绿假单胞菌在反复传代培养于浓度不断增加的两性化合物和季铵化合物中后，能够在高浓度的这两种化合物中生长。耐药性逐渐获得并丧失。对这两种杀菌剂的适应性导致对双胍类产生交叉耐药性，但适应季铵化合物的细胞对一系列季铵化合物具有抗性，而适应两性化合物的生物体则没有。适应两性化合物的细胞疏水性增加，并表现出超微结构改变，这表明外膜可能与耐药性有关。适应两性化合物和季铵化合物的生物体的脂肪酸谱都发生了变化，这与外膜修饰一致，但每种情况下的变化都不同。文中讨论了杀菌剂耐药性所涉及的机制。
