Sun Xiaolian, Huang Xinglu, Yan Xuefeng, Wang Yu, Guo Jinxia, Jacobson Orit, Liu Dingbin, Szajek Lawrence P, Zhu Wenlei, Niu Gang, Kiesewetter Dale O, Sun Shouheng, Chen Xiaoyuan
ACS Nano. 2014 Aug 26;8(8):8438-46. doi: 10.1021/nn502950t.
Using positron emission tomography (PET) imaging to monitor and quantitatively analyze the delivery and localization of Au nanomaterials (NMs), a widely used photothermal agent, is essential to optimize therapeutic protocols to achieve individualized medicine and avoid side effects. Coupling radiometals to Au NMs via a chelator faces the challenges of possible detachment of the radiometals as well as surface property changes of the NMs. In this study, we reported a simple and general chelator-free (64)Cu radiolabeling method by chemically reducing (64)Cu on the surface of polyethylene glycol (PEG)-stabilized Au NMs regardless of their shape and size. Our (64)Cu-integrated NMs are proved to be radiochemically stable and can provide an accurate and sensitive localization of NMs through noninvasive PET imaging. We further integrated (64)Cu onto arginine-glycine-aspartic acid (RGD) peptide modified Au nanorods (NRs) for tumor theranostic application. These NRs showed high tumor targeting ability in a U87MG glioblastoma xenograft model and were successfully used for PET image-guided photothermal therapy.
使用正电子发射断层扫描（PET）成像来监测和定量分析金纳米材料（NMs）（一种广泛使用的光热剂）的递送和定位，对于优化治疗方案以实现个性化医疗并避免副作用至关重要。通过螯合剂将放射性金属与金纳米材料偶联面临着放射性金属可能脱落以及纳米材料表面性质改变的挑战。在本研究中，我们报道了一种简单通用的无螯合剂（64）Cu放射性标记方法，即通过化学还原在聚乙二醇（PEG）稳定的金纳米材料表面上的（64）Cu，而不管其形状和大小如何。我们的（64）Cu整合纳米材料被证明具有放射化学稳定性，并且可以通过无创PET成像提供纳米材料的准确和灵敏定位。我们进一步将（64）Cu整合到精氨酸-甘氨酸-天冬氨酸（RGD）肽修饰的金纳米棒（NRs）上用于肿瘤诊疗应用。这些纳米棒在U87MG胶质母细胞瘤异种移植模型中显示出高肿瘤靶向能力，并成功用于PET图像引导的光热治疗。
