Daburkar Mohan, Lohar Vikram, Rathore Arvind Singh, Bhutada Pravin, Tangadpaliwar Shrikant
Department of Pharmacology, Agnihotri College of Pharmacy, Wardha, Maharashtra, India.
Faculty of Pharmaceutical Sciences, Jodhpur National University, Narnadi, Jodhpur, Rajasthan, India.
J Pharm Bioallied Sci. 2014 Jul;6(3):205-12. doi: 10.4103/0975-7406.135248.
To examine the preventive effect of Aloe vera gel ethanolic extract using diabetic foot ulcer (DFUs) protocol in Wistar rats.
Male Wistar rats were divided into untreated control (Group I), untreated DFUs (Group II), DFUs treated with A. vera gel ethanolic extract (Group III), DFUs treated with topical A. vera gel (Group IV), DFUs treated with A. vera gel ethanolic extract and topical A. vera gel (Group V). The rats in the treatment groups were daily administered the A. vera gel and ethanolic extract for 9 days. Fasting blood glucose levels and percentage of wound ulcer contraction were measured on day 3, 6, and 9.
The results are expressed as a mean ± Standard Error Mean (SEM). Data were analyzed using one-way analysis of variance (ANOVA) after Newman-Keuls test. P < 0.05 were considered statistically significant in all cases.
Oral administration of A. vera gel ethanolic extract at a dose of 300 mg/kg body weight per day to diabetic rats for a period of 9 days resulted in a significant reduction in fasting blood glucose and a significant improvement in plasma insulin. Topical application of A. vera gel at a dose 30 mg/kg body weight per day to streptozotocin (STZ)-induced diabetic rats for a period of 9 days resulted in no change in blood glucose and plasma insulin. Oral administration as well as topical application of A. vera gel ethanolic extract and gel significantly reduced the blood glucose, improved the plasma insulin, and significantly increased DNA and glycosaminoglycans (GAGs) to improve the wound ulcer healing as well as the breaking strength on day 9.
Present findings provide a scientific rationale for the use of A. vera gel ethanolic extract, and showed that the gel attenuated the diabetic foot wound in rats.
采用糖尿病足溃疡（DFUs）模型，研究芦荟凝胶乙醇提取物对Wistar大鼠的预防作用。
将雄性Wistar大鼠分为未处理对照组（I组）、未处理DFUs组（II组）、芦荟凝胶乙醇提取物处理的DFUs组（III组）、局部应用芦荟凝胶处理的DFUs组（IV组）、芦荟凝胶乙醇提取物联合局部应用芦荟凝胶处理的DFUs组（V组）。治疗组大鼠每天给予芦荟凝胶和乙醇提取物，持续9天。在第3、6和9天测量空腹血糖水平和伤口溃疡收缩百分比。
结果以平均值±标准误（SEM）表示。数据经Newman-Keuls检验后采用单因素方差分析（ANOVA）进行分析。所有情况下，P < 0.05被认为具有统计学意义。
糖尿病大鼠每天口服300 mg/kg体重的芦荟凝胶乙醇提取物，持续9天，可显著降低空腹血糖，并显著改善血浆胰岛素水平。每天对链脲佐菌素（STZ）诱导的糖尿病大鼠局部应用30 mg/kg体重的芦荟凝胶，持续9天，血糖和血浆胰岛素水平无变化。口服以及局部应用芦荟凝胶乙醇提取物和凝胶均能显著降低血糖，改善血浆胰岛素水平，并显著增加DNA和糖胺聚糖（GAGs），从而在第9天促进伤口溃疡愈合以及提高断裂强度。
目前的研究结果为芦荟凝胶乙醇提取物的应用提供了科学依据，并表明该凝胶可减轻大鼠糖尿病足伤口。
