链脲佐菌素诱导的糖尿病大鼠经提取物治疗后胰岛素分泌及胰岛β细胞功能的改善
Improvement of Insulin Secretion and Pancreatic β-cell Function in Streptozotocin-induced Diabetic Rats Treated with Extract.
作者信息
Noor Ayesha, Gunasekaran S, Vijayalakshmi M A
机构信息
Centre for Bio Separation Technology, VIT University, Vellore, Tamil Nadu, India.
Department of Physiology, Christian Medical College, Vellore, Tamil Nadu, India.
出版信息
Pharmacognosy Res. 2017 Dec;9(Suppl 1):S99-S104. doi: 10.4103/pr.pr_75_17.
BACKGROUND
Diabetes mellitus is a metabolic disorder characterized by chronic hyperglycemia. Plant extracts and their products are being used as an alternative system of medicine for the treatment of diabetes. has been traditionally used to treat several diseases and it exhibits antioxidant, anti-inflammatory, and wound-healing effects. Streptozotocin (STZ)-induced Wistar diabetic rats were used in this study to understand the potential protective effect of extract on the pancreatic islets.
OBJECTIVE
The aim of the present study was to evaluate the extract on improvement of insulin secretion and pancreatic β-cell function by morphometric analysis of pancreatic islets in STZ-induced diabetic Wistar rats.
MATERIALS AND METHODS
After acclimatization, male Wistar rats, maintained as per the Committee for the Purpose of Control and Supervision of Experiments on Animals guidelines, were randomly divided into four groups of six rats each. Fasting plasma glucose and insulin levels were assessed. The effect of extract in STZ-induced diabetic rats on the pancreatic islets by morphometric analysis was evaluated.
RESULTS
Oral administration of extract (300 mg/kg) daily to diabetic rats for 3 weeks showed restoration of blood glucose levels to normal levels with a concomitant increase in insulin levels upon feeding with extract in STZ-induced diabetic rats. Morphometric analysis of pancreatic sections revealed quantitative and qualitative gain in terms of number, diameter, volume, and area of the pancreatic islets of diabetic rats treated with extract when compared to the untreated diabetic rats.
CONCLUSION
extract exerts antidiabetic effects by improving insulin secretion and pancreatic β-cell function by restoring pancreatic islet mass in STZ-induced diabetic Wistar rats.
SUMMARY
Fasting plasma glucose (FPG) and insulin levels were restored to normal levels in diabetic rats treated with extractIslets of pancreas were qualitatively and quantitatively restored to normalcy leading to restoration of FPG and insulin levels of diabetic rats treated with extractMorphometric analysis of pancreatic sections revealed quantitative and qualitative gain in terms of number, diameter, volume, and area of the pancreatic islets of diabetic rats treated with extract when compared to the untreated diabetic rats. , FPG: Fasting plasma glucose, STZ: Streptozotocin, BW: Body weight.
背景
糖尿病是一种以慢性高血糖为特征的代谢紊乱疾病。植物提取物及其产品正被用作治疗糖尿病的替代医学体系。传统上已用于治疗多种疾病，并且具有抗氧化、抗炎和伤口愈合作用。本研究使用链脲佐菌素（STZ）诱导的Wistar糖尿病大鼠来了解提取物对胰岛的潜在保护作用。
目的
本研究的目的是通过对STZ诱导的糖尿病Wistar大鼠胰岛进行形态计量分析，评估提取物对胰岛素分泌改善和胰腺β细胞功能的影响。
材料与方法
适应环境后，按照动物实验控制与监督委员会的指南饲养雄性Wistar大鼠，将其随机分为四组，每组六只。评估空腹血糖和胰岛素水平。通过形态计量分析评估提取物对STZ诱导的糖尿病大鼠胰岛的影响。
结果
糖尿病大鼠每日口服提取物（300mg/kg），持续3周，结果显示血糖水平恢复至正常水平，同时STZ诱导的糖尿病大鼠喂食提取物后胰岛素水平随之升高。胰腺切片的形态计量分析显示，与未治疗的糖尿病大鼠相比，用提取物治疗的糖尿病大鼠胰岛在数量、直径、体积和面积方面在数量和质量上均有所增加。
结论
提取物通过恢复STZ诱导的糖尿病Wistar大鼠的胰岛质量，改善胰岛素分泌和胰腺β细胞功能，从而发挥抗糖尿病作用。
总结
用提取物治疗的糖尿病大鼠空腹血糖（FPG）和胰岛素水平恢复至正常水平胰腺胰岛在质量和数量上恢复正常，导致用提取物治疗的糖尿病大鼠FPG和胰岛素水平恢复正常胰腺切片的形态计量分析显示，与未治疗的糖尿病大鼠相比，用提取物治疗的糖尿病大鼠胰岛在数量、直径、体积和面积方面在数量和质量上均有所增加。FPG：空腹血糖，STZ：链脲佐菌素，BW：体重
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