Bhatia S J, Kochar N, Abraham P, Nair N G, Mehta A P
Department of Gastroenterology, King Edward Memorial Hospital, Bombay, India.
J Clin Microbiol. 1989 Oct;27(10):2328-30. doi: 10.1128/jcm.27.10.2328-2330.1989.
Campylobacter pylori has been implicated as a causative factor in acid-peptic disease. Lactobacillus acidophilus is known to inhibit the growth of pathogens in the human gastrointestinal tract. We recovered C. pylori from gastric antral biopsies of seven patients with acid-peptic disease; the isolates were then cultured in brucella broth. The effect of L. acidophilus (cultured in DeMan-Rogosa-Sharpe broth) on the growth of C. pylori was tested by a mixed culture technique. L. acidophilus inhibited the growth of all seven isolates of C. pylori in vitro. All these isolates were also inhibited by the L. acidophilus culture supernatant (brucella blood agar cup technique) obtained at or after 48 h of incubation. Inhibition of C. pylori growth was also observed with 1 and 3% lactic acid but not with 0.5 and 1% hydrogen peroxide, the L. acidophilus sonic extract, or a citrate-phosphate buffer (pH 4.0). We conclude that the inhibitory action of L. acidophilus on C. pylori is dependent on an extracellular secretory product, probably lactic acid. This inhibitory effect may be of therapeutic relevance in patients with C. pylori-positive acid-peptic disease.
幽门螺杆菌被认为是酸相关性疾病的致病因素。已知嗜酸乳杆菌可抑制人类胃肠道中病原体的生长。我们从7例酸相关性疾病患者的胃窦活检组织中分离出幽门螺杆菌；然后将分离株在布氏肉汤中培养。采用混合培养技术检测嗜酸乳杆菌（在德氏乳杆菌-罗格斯-夏普肉汤中培养）对幽门螺杆菌生长的影响。嗜酸乳杆菌在体外抑制了所有7株幽门螺杆菌的生长。所有这些分离株也受到在培养48小时及以后获得的嗜酸乳杆菌培养上清液（布氏血琼脂杯碟法）的抑制。用1%和3%的乳酸也观察到了对幽门螺杆菌生长的抑制作用，但用0.5%和1%的过氧化氢、嗜酸乳杆菌超声提取物或柠檬酸盐-磷酸盐缓冲液（pH 4.0）未观察到抑制作用。我们得出结论，嗜酸乳杆菌对幽门螺杆菌的抑制作用取决于一种细胞外分泌产物，可能是乳酸。这种抑制作用可能对幽门螺杆菌阳性的酸相关性疾病患者具有治疗意义。
