Brossard Stoos Kari A, Ren Jennifer, Shields-Cutler Robin R, Sams Kelly L, Caldwell Shannon, Ho Marvin B, Rivara Gregg, Whistler Cheryl A, Jones Stephen H, Wiedmann Martin, DeMent Jamie, Getchell Rodman G, Marquis Hélène
Department of Microbiology and Immunology, Cornell University, Ithaca, NY, United States.
Department of Health Promotion and Physical Education, Ithaca College, Ithaca, NY, United States.
Front Microbiol. 2022 Dec 19;13:1041942. doi: 10.3389/fmicb.2022.1041942. eCollection 2022.
Gastrointestinal illnesses associated with the consumption of shellfish contaminated with have a negative impact on the shellfish industry due to recalls and loss of consumer confidence in products. This bacterial pathogen is very diverse and specific sequence types (STs), ST631 and ST36, have emerged as prevalent causes of foodborne disease outbreaks in the US, though other STs have been implicated in sporadic cases. We investigated whether bacteriophages could be used as a proxy to monitor for the presence of distinct STs in coastal waters.
For this purpose, bacteriophages infecting were isolated from water samples collected on the Northeast Atlantic coast. The isolated phages were tested against a collection of 29 isolates representing 18 STs, including six clonal complexes (CC). Four distinct phages were identified based on their ability to infect different sets of isolates.
Overall, the 29 bacterial isolates segregated into one of eight patterns of susceptibility, ranging from resistance to all four phages to susceptibility to any number of phages. STs represented by more than one bacterial isolate segregated within the same pattern of susceptibility except for one ST. Other patterns of susceptibility included exclusively clinical isolates represented by distinct STs. Overall, this study suggests that phages populating coastal waters could be exploited to monitor for the presence of STs known to cause foodborne outbreaks.
食用受污染贝类导致的胃肠道疾病，因产品召回以及消费者对产品信心丧失，对贝类产业产生负面影响。这种细菌病原体具有高度多样性，特定序列类型（STs），即ST631和ST36，已成为美国食源性疾病暴发的常见原因，不过其他STs也与散发病例有关。我们调查了噬菌体是否可作为监测沿海水域中不同STs存在情况的替代指标。
为此，从东北大西洋沿岸采集的水样中分离出感染的噬菌体。将分离出的噬菌体针对一组代表18种STs的29株分离株进行测试，其中包括六个克隆复合体（CC）。根据其感染不同分离株的能力，鉴定出四种不同的噬菌体。
总体而言，29株细菌分离株分为八种敏感性模式之一，范围从对所有四种噬菌体均耐药到对任意数量的噬菌体敏感。除一种ST外，由多个细菌分离株代表的STs在相同的敏感性模式内分类。其他敏感性模式仅包括由不同STs代表的临床分离株。总体而言，本研究表明，可利用沿海水域中的噬菌体来监测已知会导致食源性暴发的STs的存在情况。
