Chandler-Bostock Rebecca, Hancox Laura R, Nawaz Sameena, Watts Oliver, Iturriza-Gomara Miren, Mellits Kenneth H
University of Nottingham, School of Biosciences, Division of Food Science, Sutton Bonington Campus, Loughborough LE12 5RD, UK.
Virus Reference Department, Public Health England, London, NW9 5HT, UK.
Vet Microbiol. 2014 Sep 17;173(1-2):27-37. doi: 10.1016/j.vetmic.2014.06.030. Epub 2014 Jul 8.
Rotavirus is endemic in pig farms where it causes a loss in production. This study is the first to characterise porcine rotavirus circulating in UK pigs. Samples from diarrheic pigs with rotavirus enteritis obtained between 2010 and 2012 were genotyped in order to determine the diversity of group A rotavirus (GARV) in UK pigs. A wide range of rotavirus genotypes were identified in UK pigs: six G types (VP7); G2, G3, G4, G5, G9 and G11 and six P types (VP4); P[6], P[7], P[8], P[13], P[23], and P[32]. With the exception of a single P[8] isolate, there was less than 95% nucleotide identity between sequences from this study and any available rotavirus sequences. The G9 and P[6] genotypes are capable of infecting both humans and pigs, but showed no species cross-over within the UK as they were shown to be genetically distinct, which suggested zoonotic transmission is rare within the UK. We identified the P[8] genotype in one isolate, this genotype is almost exclusively found in humans. The P[8] was linked to a human Irish rotavirus isolate in the same year. The discovery of human genotype P[8] rotavirus in a UK pig confirms this common human genotype can infect pigs and also highlights the necessity of surveillance of porcine rotavirus genotypes to safeguard human as well as porcine health.
轮状病毒在养猪场呈地方性流行，可导致生产损失。本研究首次对在英国猪群中传播的猪轮状病毒进行了特征描述。对2010年至2012年间从患有轮状病毒肠炎的腹泻猪中采集的样本进行基因分型，以确定英国猪群中A组轮状病毒（GARV）的多样性。在英国猪群中鉴定出了多种轮状病毒基因型：六种G型（VP7）；G2、G3、G4、G5、G9和G11，以及六种P型（VP4）；P[6]、P[7]、P[8]、P[13]、P[23]和P[32]。除了一个P[8]分离株外，本研究的序列与任何可用的轮状病毒序列之间的核苷酸同一性均低于95%。G9和P[6]基因型能够感染人类和猪，但在英国未显示出种间交叉，因为它们在基因上是不同的，这表明在英国人畜共患传播很少见。我们在一个分离株中鉴定出了P[8]基因型，该基因型几乎只在人类中发现。该P[8]与同年一株爱尔兰人类轮状病毒分离株相关。在英国猪中发现人类基因型P[8]轮状病毒证实了这种常见的人类基因型可以感染猪，也凸显了监测猪轮状病毒基因型以保障人类和猪健康的必要性。
