通过聚合酶链反应检测HTLV-I血清学阳性母亲所生血清学阴性婴儿中的HTLV-I基因组。
Detection of HTLV-I genome in seronegative infants born to HTLV-I seropositive mothers by polymerase chain reaction.
作者信息
Saito S, Ando Y, Furuki K, Kakimoto K, Tanigawa T, Moriyama I, Ichijo M, Nakamura M, Ohtani K, Sugamura K
机构信息
Department of Obstetrics and Gynecology, Nara Medical University.
出版信息
Jpn J Cancer Res. 1989 Sep;80(9):808-12. doi: 10.1111/j.1349-7006.1989.tb01718.x.
Abstract
We applied the polymerase chain reaction (PCR) method to detect gag, env and pX sequences of human T cell leukemia virus type I (HTLV-I) provirus in peripheral blood lymphocytes of seronegative infants born to HTLV-I seropositive mothers. Out of 22, five subjects were found to contain the HTLV-I provirus genome. Two of the five cases were judged to be negative for not only anti-HTLV-I antibodies but also the viral antigens on cultivated lymphocytes by the conventional antibody/antigen detection methods. These results indicate that PCR is of great use as a simple and highly sensitive method detect HTLV-I infection.
摘要
我们应用聚合酶链反应（PCR）方法检测人类嗜T淋巴细胞病毒I型（HTLV-I）血清反应阴性婴儿外周血淋巴细胞中HTLV-I前病毒的gag、env和pX序列，这些婴儿的母亲为HTLV-I血清反应阳性。在22名婴儿中，有5名被发现含有HTLV-I前病毒基因组。通过传统的抗体/抗原检测方法，这5例中的2例不仅抗HTLV-I抗体呈阴性，而且培养淋巴细胞上的病毒抗原也呈阴性。这些结果表明，PCR作为一种简单且高度灵敏的检测HTLV-I感染的方法具有很大用途。
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本文引用的文献
1
Adult T-cell leukemia: antigen in an ATL cell line and detection of antibodies to the antigen in human sera.成人T细胞白血病：一种成人T细胞白血病细胞系中的抗原及人血清中该抗原抗体的检测
Proc Natl Acad Sci U S A. 1981 Oct;78(10):6476-80. doi: 10.1073/pnas.78.10.6476.
2
Identification of a glycoprotein, gp21, of adult T cell leukemia virus by monoclonal antibody.用单克隆抗体鉴定成人T细胞白血病病毒的一种糖蛋白gp21
J Immunol. 1984 Jun;132(6):3180-4.
3
Human adult T-cell leukemia virus: complete nucleotide sequence of the provirus genome integrated in leukemia cell DNA.人类成人T细胞白血病病毒：整合于白血病细胞DNA中的前病毒基因组的完整核苷酸序列。
Proc Natl Acad Sci U S A. 1983 Jun;80(12):3618-22. doi: 10.1073/pnas.80.12.3618.
4
Detection and isolation of type C retrovirus particles from fresh and cultured lymphocytes of a patient with cutaneous T-cell lymphoma.从一名皮肤T细胞淋巴瘤患者的新鲜淋巴细胞和培养淋巴细胞中检测并分离C型逆转录病毒颗粒。
Proc Natl Acad Sci U S A. 1980 Dec;77(12):7415-9. doi: 10.1073/pnas.77.12.7415.
5
Monoclonal antibody reactive with both p28 and p19 of adult T-cell leukemia virus-specific polypeptides.
Gan. 1983 Jun;74(3):327-30.
6
Primary infection of Japanese infants with adult T-cell leukaemia-associated retrovirus (ATLV): evidence for viral transmission from mothers to children.
J Infect. 1986 May;12(3):205-12. doi: 10.1016/s0163-4453(86)94086-7.
7
Mother-to-child transmission of human T-cell leukemia virus type-I.人类I型T细胞白血病病毒的母婴传播。
Jpn J Cancer Res. 1985 Jun;76(6):474-80.
8
Identification of HIV-infected seronegative individuals by a direct diagnostic test based on hybridisation to amplified viral DNA.通过基于与扩增病毒DNA杂交的直接诊断测试来鉴定HIV感染的血清阴性个体。
Lancet. 1988 Aug 20;2(8608):418-21. doi: 10.1016/s0140-6736(88)90412-6.
9
Transmission of adult T-cell leukemia retrovirus (HTLV-I) from mother to child: comparison of bottle- with breast-fed babies.
Jpn J Cancer Res. 1987 Apr;78(4):322-4.
10
Prevention of HTLV-I transmission through the breast milk by a freeze-thawing process.
Jpn J Cancer Res. 1986 Oct;77(10):974-7.
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