Hjelle B, Mills R, Goldsmith C, Swenson S G, Cyrus S
Department of Pathology, University of New Mexico School of Medicine, Albuquerque 87131.
J Clin Microbiol. 1992 Aug;30(8):2195-9. doi: 10.1128/jcm.30.8.2195-2199.1992.
We describe the use of an immunofluorescence assay and coculture to confirm human T-cell leukemia-lymphoma virus (HTLV) infection. Peripheral blood mononuclear cells from 32 of 32 seropositive donors were positive in the immunofluorescence assay, and 63% of their cocultures produced p24 antigen. Specific antibodies distinguished HTLV type I (HTLV-I) from HTLV-II. HTLV-I or HTLV-II was isolated from donors with indeterminate serologic test results.
我们描述了使用免疫荧光测定法和共培养来确认人类T细胞白血病-淋巴瘤病毒(HTLV)感染的情况。32名血清反应阳性供者的外周血单个核细胞在免疫荧光测定中呈阳性,并且其共培养物中有63%产生了p24抗原。特异性抗体可区分I型HTLV(HTLV-I)和II型HTLV(HTLV-II)。从血清学检测结果不确定的供者中分离出了HTLV-I或HTLV-II。