Berens Christian, Suess Beatrix
Fachbereich Biologie, Technische Universität Darmstadt, Schnittspahnstraße 10, 64287 Darmstadt, Germany.
Curr Opin Biotechnol. 2015 Feb;31:10-5. doi: 10.1016/j.copbio.2014.07.014. Epub 2014 Aug 17.
Synthetic biology uses our understanding of biological systems to develop innovative solutions for challenges in fields as diverse as genetic control and logic devices, bioremediation, materials production or diagnostics and therapy in medicine by designing new biological components. RNA-based elements are key components of these engineered systems. Their structural and functional diversity is ideal for generating regulatory riboswitches that react with many different types of output to molecular and environmental signals. Recent advances have added new sensor and output domains to the existing toolbox, and demonstrated the portability of riboswitches to many different organisms. Improvements in riboswitch design and screens for selecting in vivo active switches provide the means to isolate riboswitches with regulatory properties more like their natural counterparts.
合成生物学利用我们对生物系统的理解，通过设计新的生物组件，为基因控制与逻辑装置、生物修复、材料生产以及医学诊断与治疗等诸多不同领域的挑战开发创新解决方案。基于RNA的元件是这些工程系统的关键组件。它们的结构和功能多样性非常适合生成与分子和环境信号的许多不同类型输出发生反应的调控核糖开关。最近的进展为现有工具箱增添了新的传感器和输出结构域，并证明了核糖开关在许多不同生物体中的可移植性。核糖开关设计的改进以及用于筛选体内活性开关的方法，为分离具有更类似于其天然对应物调控特性的核糖开关提供了手段。
