Rickard J P, Schmidt R E, Maddison J W, Bathgate R, Lynch G W, Druart X, de Graaf S P
RMC Gunn Building, Regimental Drive, Faculty of Veterinary Science, The University of Sydney, NSW 2006, Australia.
UMR 6175 INRA, CNRS-Université de Tours-Haras Nationaux, Station de Physiologie de la Reproduction et des Comportements, Institut National de la Recherche Agronomique, 37380 Nouzilly, France.
Reprod Fertil Dev. 2016 Mar;28(4):516-23. doi: 10.1071/RD14123.
Variation in the effect of seminal plasma on sperm function and fertility has been hypothesised to be due to differences between males and their seminal plasma composition. The freezing resilience of individual rams (n=17) was investigated to characterise inter-male variation. This was determined by measuring the degree of change in motility induced by cryopreservation (Experiment 1). Experiment 2 examined the effect of pooled seminal plasma from rams identified as having high or low resilience to freezing on the cryosurvival of washed spermatozoa from either high (n=3) or low (n=3) sperm freezing resilience rams. Immediately after thawing and throughout the incubation period (0-4h), spermatozoa from high-resilience rams frozen with high-resilience seminal plasma demonstrated superior motility to spermatozoa from high-resilience rams frozen with low-resilience seminal plasma (P<0.001). Similarly, spermatozoa from low-resilience rams frozen with high-resilience seminal plasma exhibited higher motility than spermatozoa from low-resilience rams frozen with low-resilience seminal plasma immediately after thawing (0h; P<0.001). The present study shows that variation in freezing resilience of ram spermatozoa is related to the source and composition of the seminal plasma.
精液对精子功能和生育能力影响的差异被认为是由于雄性个体及其精液成分不同所致。研究了17只公羊的冻存恢复力，以表征雄性个体间的差异。这是通过测量冷冻保存引起的活力变化程度来确定的（实验1）。实验2研究了从具有高或低冷冻恢复力的公羊中收集的精液对来自高（n = 3）或低（n = 3）精子冷冻恢复力公羊的洗涤精子冷冻存活率的影响。解冻后立即及整个孵育期（0 - 4小时），用高恢复力精液冷冻的高恢复力公羊精子的活力优于用低恢复力精液冷冻的高恢复力公羊精子（P < 0.001）。同样，解冻后立即（0小时；P < 0.001），用高恢复力精液冷冻的低恢复力公羊精子的活力高于用低恢复力精液冷冻的低恢复力公羊精子。本研究表明，公羊精子冷冻恢复力的差异与精液的来源和成分有关。
