通过1H检测的固态核磁共振对麻疹病毒核衣壳的结构与动力学的深入研究
Insights into the structure and dynamics of measles virus nucleocapsids by 1H-detected solid-state NMR.
作者信息
Barbet-Massin Emeline, Felletti Michele, Schneider Robert, Jehle Stefan, Communie Guillaume, Martinez Nicolas, Jensen Malene Ringkjøbing, Ruigrok Rob W H, Emsley Lyndon, Lesage Anne, Blackledge Martin, Pintacuda Guido
机构信息
Centre de RMN à Très Hauts Champs, Institut des Sciences Analytiques, UMR 5280 CNRS/Ecole Normale Supérieure de Lyon/UCBL, University of Lyon, 69100 Villeurbanne, France.
Institut de Biologie Structurale, CEA, CNRS, UJF, 38027 Grenoble, France.
出版信息
Biophys J. 2014 Aug 19;107(4):941-6. doi: 10.1016/j.bpj.2014.05.048.
Abstract
(1)H-detected solid-state nuclear magnetic resonance (NMR) experiments are recorded on both intact and trypsin-cleaved sedimented measles virus (MeV) nucleocapsids under ultra-fast magic-angle spinning. High-resolution (1)H,(15)N-fingerprints allow probing the degree of molecular order and flexibility of individual capsid proteins, providing an exciting atomic-scale complement to electro microscopy (EM) studies of the same systems.
摘要
（1）氢检测固态核磁共振（NMR）实验是在超快速魔角旋转条件下，对完整的和经胰蛋白酶切割的沉降麻疹病毒（MeV）核衣壳进行记录的。高分辨率的氢-氮指纹图谱能够探测单个衣壳蛋白的分子有序度和灵活性，为同一系统的电子显微镜（EM）研究提供了令人兴奋的原子尺度补充。
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