Ebrahimi Azizollah, Ghasemi Maryam, Ghasemi Bahram
Institute of Zoonotic Diseases, School of Veterinary Science, Shahrekord University, Shahrekord, IR Iran.
School of Veterinary Science, Shahrekord University, Shahrekord, IR Iran.
Jundishapur J Microbiol. 2014 Apr;7(4):e9225. doi: 10.5812/jjm.9225. Epub 2014 Apr 1.
Staphylococci release a large number of enzymes. Some of these, such as coagulase, beta- lactamase, hemolysins and biofilms are considered indices of pathogenicity.
The aim of the current study was based on the isolation and identification of Staphylococcus aureus and coagulase negative Staphylococci (CNS) strains from various skin lesions and examining their biofilms, beta- lactamase, hemolysins production and antibiotic resistance pattern.
Sixty one infected wounds and 39 skin infections samples were collected and examined. After the culture and identification, examination for production of hemolysins, beta- lactamase, biofilm and susceptibility toward 9 antimicrobials was performed.
Out of 75 isolated Staphylococci, sixty (80%) were biofilm producers. Two overall prevalence of 28.5% and 100% of ß-lactamase production were recorded for isolated S. aureus and CNS, respectively. Twenty out of 49 (40.8%), the same number of α- and β- hemolytic S. aureus, were isolated while six (12.24%) were ∂ -hemolysin producers. Twenty two of Twenty six (84.6%) isolates of CNS, were hemolysin producers that all were ∂ type. The S. aureus isolates from wound infections, show a high sensitivity pattern to all examined antibiotics, this sensitivity pattern for isolates from skin dermatitis is relatively low, though.
High percentage of hemolysins, biofilm and beta lactamase production by isolated Staphylococci, suggests an important role of these virulence factors in the pathogenesis of isolated Staphylococci from dermatitis lesions. The S. aureus isolates from wound infections, show a high sensitivity pattern to all examined antibiotics. Only ciprofloxacin was found to be active against all isolates from dedermatitis lesions.
葡萄球菌可释放大量酶类。其中一些酶，如凝固酶、β-内酰胺酶、溶血素和生物膜，被视为致病性指标。
本研究旨在从各种皮肤损伤中分离和鉴定金黄色葡萄球菌及凝固酶阴性葡萄球菌（CNS）菌株，并检测它们的生物膜、β-内酰胺酶、溶血素产生情况及抗生素耐药模式。
收集并检测61份感染伤口样本和39份皮肤感染样本。经培养和鉴定后，检测溶血素、β-内酰胺酶、生物膜的产生情况以及对9种抗菌药物的敏感性。
在75株分离出的葡萄球菌中，60株（80%）产生生物膜。分离出的金黄色葡萄球菌和CNS中，β-内酰胺酶产生的总体患病率分别为28.5%和100%。49株（40.8%）金黄色葡萄球菌中分离出20株，α-溶血和β-溶血的金黄色葡萄球菌数量相同，同时有6株（12.24%）产生δ-溶血素。26株CNS分离株中有22株（84.6%）产生溶血素，均为δ型。伤口感染分离出的金黄色葡萄球菌对所有检测抗生素均表现出高敏感性，而皮肤性皮炎分离株的这种敏感性相对较低。
分离出的葡萄球菌产生溶血素、生物膜和β-内酰胺酶的比例较高，表明这些毒力因子在皮炎病变分离出的葡萄球菌发病机制中起重要作用。伤口感染分离出的金黄色葡萄球菌对所有检测抗生素均表现出高敏感性。仅发现环丙沙星对所有皮肤炎病变分离株均有活性。
