Zhang Weijie, Lian Qin, Li Dichen, Wang Kunzheng, Hao Dingjun, Bian Weiguo, He Jiankang, Jin Zhongmin
State Key Laboratory for Manufacturing Systems Engineering, Xi'an Jiaotong University, Xi'an 710054, China ; The First Department of Orthopaedics, The Second Affiliated Hospital, Health Science Center, Xi'an Jiaotong University, Xi'an 710004, China ; Department of Joint Surgery, Hong Hui Hospital, Health Science Center, Xi'an Jiaotong University, Xi'an 710054, China.
State Key Laboratory for Manufacturing Systems Engineering, Xi'an Jiaotong University, Xi'an 710054, China.
Biomed Res Int. 2014;2014:746138. doi: 10.1155/2014/746138. Epub 2014 Aug 7.
Increasing evidences show that subchondral bone may play a significant role in the repair or progression of cartilage damage in situ. However, the exact change of subchondral bone during osteochondral repair is still poorly understood. In this paper, biphasic osteochondral composite scaffolds were fabricated by 3D printing technology using PEG hydrogel and β-TCP ceramic and then implanted in rabbit trochlea within a critical size defect model. Animals were euthanized at 1, 2, 4, 8, 16, 24, and 52 weeks after implantation. Histological results showed that hyaline-like cartilage formed along with white smooth surface and invisible margin at 24 weeks postoperatively, typical tidemark formation at 52 weeks. The repaired subchondral bone formed from 16 to 52 weeks in a "flow like" manner from surrounding bone to the defect center gradually. Statistical analysis illustrated that both subchondral bone volume and migration area percentage were highly correlated with the gross appearance Wayne score of repaired cartilage. Therefore, subchondral bone migration is related to cartilage repair for critical size osteochondral defects. Furthermore, the subchondral bone remodeling proceeds in a "flow like" manner and repaired cartilage with tidemark implies that the biphasic PEG/β-TCP composites fabricated by 3D printing provides a feasible strategy for osteochondral tissue engineering application.
越来越多的证据表明，软骨下骨可能在原位软骨损伤的修复或进展中发挥重要作用。然而，在骨软骨修复过程中软骨下骨的确切变化仍知之甚少。在本文中，采用聚乙二醇水凝胶和β - 磷酸三钙陶瓷通过3D打印技术制备了双相骨软骨复合支架，然后将其植入兔滑车的临界尺寸缺损模型中。在植入后1、2、4、8、16、24和52周对动物实施安乐死。组织学结果显示，术后24周形成了具有白色光滑表面和不可见边缘的类透明软骨，52周时形成了典型的潮标。修复的软骨下骨在16至52周内以“流动状”方式从周围骨逐渐向缺损中心形成。统计分析表明，软骨下骨体积和迁移面积百分比均与修复软骨的大体外观韦恩评分高度相关。因此，软骨下骨迁移与临界尺寸骨软骨缺损的软骨修复有关。此外，软骨下骨重塑以“流动状”方式进行，带有潮标的修复软骨表明通过3D打印制备的双相聚乙二醇/β - 磷酸三钙复合材料为骨软骨组织工程应用提供了一种可行的策略。
