单瓣暗罗种子富含黄酮部分对白化大鼠的急性和亚急性经口毒性研究
Acute and subacute oral toxicity study on the flavonoid rich fraction of Monodora tenuifolia seed in albino rats.
作者信息
Ekeanyanwu Raphael Chukwuma, Njoku Obioma Uzoma
机构信息
Department of Biochemistry, Faculty of Sciences, Imo State University Owerri, Imo State, Nigeria.
Department of Biochemistry, Faculty of Biological Sciences, University of Nigeria Nsukka, Enugu State, Nigeria.
出版信息
Asian Pac J Trop Biomed. 2014 Mar;4(3):194-202. doi: 10.1016/S2221-1691(14)60231-8.
OBJECTIVE
To investigate the effects of the flavonoid rich fraction of Monodora tenuifolia seed on the haematology, histopathology and liver profile of Wistar albino rats.
METHODS
Toxicity study was investigated on the flavonoid rich fraction of Monodora tenuifolia in rats administered different concentrations orally for 28 d using standard laboratory procedures.
RESULTS
The LD50 of the flavonoid rich fraction was found to be above 5 000 mg/kg body weight in mice observed for 48 h. After the Day 14, biochemical markers of liver injury such as serum alanine aminotransferase, and aspartate aminotransferase decreased significantly (P<0.05 at doses of 100 and 200 mg/kg body weight and P<0.01 at 400 mg/kg) while serum alkaline phosphatase increased non-significantly (P>0.05). There was non-significant (P>0.05) effect observed across the groups in the levels of serum total protein, albumin, globulin, urea and creatinine. The result of histological examination showed various degrees of peribiliary hepatitis after the Day 14 which fizzled out after the Day 28.
CONCLUSIONS
The result therefore suggests that the seed extract is potentially safe.
目的
研究薄叶单室木兰种子富含黄酮部分对Wistar白化大鼠血液学、组织病理学和肝脏指标的影响。
方法
采用标准实验室程序，对不同浓度口服给药28天的大鼠进行薄叶单室木兰富含黄酮部分的毒性研究。
结果
在观察48小时的小鼠中，富含黄酮部分的半数致死量（LD50）高于5000mg/kg体重。第14天后，肝脏损伤的生化指标如血清丙氨酸氨基转移酶和天冬氨酸氨基转移酶显著降低（体重剂量为100和200mg/kg时P<0.05，400mg/kg时P<0.01），而血清碱性磷酸酶无显著升高（P>0.05）。各组血清总蛋白、白蛋白、球蛋白、尿素和肌酐水平无显著（P>0.05）影响。组织学检查结果显示，第14天后出现不同程度的胆管周围肝炎，第28天后逐渐消退。
结论
因此，结果表明该种子提取物可能是安全的。
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