Hayleeyesus Samuel Fekadu, Manaye Abayneh Melaku
Department of Environmental Health Science and Technology, College of Public Health and Medical Science, Jimma University, Ethiopia.
Asian Pac J Trop Biomed. 2014 May;4(Suppl 1):S312-7. doi: 10.12980/APJTB.4.2014C807.
To evaluate the concentration of bacteria and fungi in the indoor environment of Jimma University libraries, so as to estimate the health hazard and to create standards for indoor air quality control.
The microbial quality of indoor air of eight libraries of Jimma University was determined. The settle plate method using open Petri-dishes containing different culture media was employed to collect sample twice daily. Isolates were identified according to standard methods.
The concentrations of bacteria and fungi aerosols in the indoor environment of the university libraries ranged between 367-2595 CFU/m(3). According to the sanitary standards classification of European Commission, almost all the libraries indoor air of Jimma University was heavily contaminated with bacteria and fungi. In spite of their major source difference, the average fungi density found in the indoor air of libraries did appear to follow the same trend with bacterial density (P=0.001). The bacteria isolates included Micrococcus sp., Staphylococcus aureus, Streptococcus pyogenes, Bacillus sp. and Neisseria sp. while Cladosporium sp., Alternaria sp., Penicillium sp. and Aspergillus sp. were the most isolated fungi.
The indoor air of all libraries were in the range above highly contaminated according to European Commission classification and the most isolates are considered as potential candidates involved in the establishment of sick building syndromes and often associated with clinical manifestations like allergy, rhinitis, asthma and conjunctivitis. Thus, attention must be given to control those environmental factors which favor the growth and multiplication of microbes in indoor environment of libraries to safeguard the health of users and workers.
评估吉姆马大学图书馆室内环境中的细菌和真菌浓度，以估计健康危害并制定室内空气质量控制标准。
测定了吉姆马大学八个图书馆室内空气的微生物质量。采用使用装有不同培养基的开放培养皿的沉降平板法，每天采集两次样本。分离株根据标准方法进行鉴定。
大学图书馆室内环境中细菌和真菌气溶胶的浓度在367 - 2595 CFU/m³之间。根据欧盟委员会的卫生标准分类，吉姆马大学几乎所有图书馆的室内空气都受到细菌和真菌的严重污染。尽管它们的主要来源不同，但图书馆室内空气中发现的平均真菌密度与细菌密度似乎遵循相同的趋势（P = 0.001）。细菌分离株包括微球菌属、金黄色葡萄球菌、化脓性链球菌、芽孢杆菌属和奈瑟菌属，而枝孢菌属、链格孢属、青霉属和曲霉属是最常分离出的真菌。
根据欧盟委员会的分类，所有图书馆的室内空气都处于高度污染以上的范围，并且大多数分离株被认为是参与病态建筑综合征形成的潜在因素，并且常常与过敏、鼻炎、哮喘和结膜炎等临床表现相关。因此，必须注意控制那些有利于图书馆室内环境中微生物生长和繁殖的环境因素，以保障使用者和工作人员的健康。
