Chavez-Santoscoy Rocio A, Gutierrez-Uribe Janet A, Granados Omar, Torre-Villalvazo Ivan, Serna-Saldivar Sergio O, Torres Nimbe, Palacios-González Berenice, Tovar Armando R
Centro de Biotecnología FEMSA, Escuela de Biotecnología y Alimentos, Tecnológico de Monterrey, Campus Monterrey,Avenida Eugenio Garza Sada 2501 Sur,C.P. 64849Monterrey, NL,Mexico.
Departamento de Fisiología de la Nutrición,Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán,Vasco de Quiroga No. 15,C.P. 14000Mexico, DF,Mexico.
Br J Nutr. 2014 Sep 28;112(6):886-99. doi: 10.1017/S0007114514001536.
Black bean (Phaseolus vulgaris L.) seed coats are a rich source of natural compounds with potential beneficial effects on human health. Beans exert hypolipidaemic activity; however, this effect has not been attributed to any particular component, and the underlying mechanisms of action and protein targets remain unknown. The aim of the present study was to identify and quantify primary saponins and flavonoids extracted from black bean seed coats, and to study their effects on lipid metabolism in primary rat hepatocytes and C57BL/6 mice. The methanol extract of black bean seed coats, characterised by a HPLC system with a UV-visible detector and an evaporative light-scattering detector and HPLC-time-of-flight/MS, contained quercetin 3-O-glucoside and soyasaponin Af as the primary flavonoid and saponin, respectively. The extract significantly reduced the expression of SREBP1c, FAS and HMGCR, and stimulated the expression of the reverse cholesterol transporters ABCG5/ABCG8 and CYP7A1 in the liver. In addition, there was an increase in the expression of hepatic PPAR-α. Consequently, there was a decrease in hepatic lipid depots and a significant increase in bile acid secretion. Furthermore, the ingestion of this extract modulated the proportion of lipids that was used as a substrate for energy generation. Thus, the results suggest that the extract of black bean seed coats may decrease hepatic lipogenesis and stimulate cholesterol excretion, in part, via bile acid synthesis.
黑豆（菜豆）种皮富含对人体健康具有潜在益处的天然化合物。豆类具有降血脂活性；然而，这种作用尚未归因于任何特定成分，其潜在的作用机制和蛋白质靶点仍不清楚。本研究的目的是鉴定和定量从黑豆种皮中提取的主要皂苷和黄酮类化合物，并研究它们对原代大鼠肝细胞和C57BL/6小鼠脂质代谢的影响。采用配有紫外可见检测器和蒸发光散射检测器的高效液相色谱系统以及高效液相色谱-飞行时间质谱对黑豆种皮的甲醇提取物进行分析，结果表明，提取物中主要的黄酮类化合物为槲皮素3-O-葡萄糖苷，主要的皂苷为大豆皂苷Af。该提取物显著降低了肝脏中SREBP1c、FAS和HMGCR的表达，并刺激了逆向胆固醇转运蛋白ABCG5/ABCG8和CYP7A1的表达。此外，肝脏中PPAR-α的表达增加。因此，肝脏脂质储存减少，胆汁酸分泌显著增加。此外，摄入该提取物调节了用作能量生成底物的脂质比例。因此，结果表明黑豆种皮提取物可能部分通过胆汁酸合成减少肝脏脂肪生成并刺激胆固醇排泄。
