Department of Bioengineering, California Institute of Technology, Pasadena, California 91125, USA.
1] Department of Bioengineering, California Institute of Technology, Pasadena, California 91125, USA [2] Department of Computer Science, California Institute of Technology, Pasadena, California 91125, USA [3] Department of Computation &Neural Systems, California Institute of Technology, Pasadena, California 91125, USA.
Nat Commun. 2014 Sep 10;5:4889. doi: 10.1038/ncomms5889.
DNA origami has proven useful for organizing diverse nanoscale components into patterns with 6 nm resolution. However for many applications, such as nanoelectronics, large-scale organization of origami into periodic lattices is desired. Here, we report the self-assembly of DNA origami rectangles into two-dimensional lattices based on stepwise control of surface diffusion, implemented by changing the concentrations of cations on the surface. Previous studies of DNA–mica binding identified the fractional surface density of divalent cations (ñ(s2))as the parameter which best explains the behaviour of linear DNA on mica. We show that for ñ(s2) between 0.04 and 0.1, over 90% of DNA rectangles were incorporated into lattices and that, compared with other functions of cation concentration, ñ(s2) best captures the behaviour of DNA rectangles. This work shows how a physical understanding of DNA–mica binding can be used to guide studies of the higher-order assembly of DNA nanostructures, towards creating large-scale arrays of nanodevices for technology.
DNA 折纸术已被证明可用于将各种纳米级组件组织成具有 6nm 分辨率的图案。然而，对于许多应用，如纳米电子学，需要将折纸术大规模地组织成周期性晶格。在这里，我们报告了 DNA 折纸矩形通过逐步控制表面扩散，通过改变表面上阳离子的浓度，自组装成二维晶格。以前的 DNA-云母结合研究确定了二价阳离子的分数表面密度（ñ(s2)) 作为最佳解释线性 DNA 在云母上行为的参数。我们表明，对于 0.04 到 0.1 之间的ñ(s2)，超过 90%的 DNA 矩形被纳入晶格中，并且与阳离子浓度的其他函数相比，ñ(s2)最佳地捕获了 DNA 矩形的行为。这项工作展示了如何利用对 DNA-云母结合的物理理解来指导 DNA 纳米结构的高级组装研究，以创建用于技术的大规模纳米器件阵列。
