Physik-Department E14 and ZNN/WSI, TU München, Am Coulombwall 4a, 85748 Garching (Germany).
Angew Chem Int Ed Engl. 2014 Jul 14;53(29):7665-8. doi: 10.1002/anie.201403965. Epub 2014 Jun 4.
The arrangement of DNA-based nanostructures into extended higher order assemblies is an important step towards their utilization as functional molecular materials. We herein demonstrate that by electrostatically controlling the adhesion and mobility of DNA origami structures on mica surfaces by the simple addition of monovalent cations, large ordered 2D arrays of origami tiles can be generated. The lattices can be formed either by close-packing of symmetric, non-interacting DNA origami structures, or by utilizing blunt-end stacking interactions between the origami units. The resulting crystalline lattices can be readily utilized as templates for the ordered arrangement of proteins.
将基于 DNA 的纳米结构排列成扩展的高级组装体是将其用作功能分子材料的重要步骤。在此，我们证明通过简单地添加单价阳离子来静电控制 DNA 折纸结构在云母表面上的粘附和迁移率，可以生成大型有序的 2D 折纸瓦片阵列。这些晶格可以通过紧密堆积对称的、非相互作用的 DNA 折纸结构形成，或者通过利用折纸单元之间的钝端堆积相互作用形成。所得的结晶晶格可以很容易地用作蛋白质有序排列的模板。
