• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

采用液相色谱-电喷雾串联质谱法（LC-ESI-MS(n)）和液相色谱-二极管阵列检测法（LC-DAD）对中药丹木注射液中的主要成分进行定性和定量分析。

Qualitative and quantitative analysis of the major constituents in traditional Chinese medicine Danmu injection using LC-ESI-MS(n) and LC-DAD.

作者信息

Zhu Fen-Xia, Wang Jing-Jing, Li Xiu-Feng, Sun E, Jia Xiao-Bin

机构信息

Key Laboratory of New Drug Delivery System of Chinese Meteria Medica, Jiangsu Provincial Academy of Chinese Medicine, Nanjing 210028, P.R. China.

Engineering Centers for Li Nationality Medicine, Hainan Pharmaceutical Factory, Co., Ltd, Wuzhishan 572200, P.R. China.

出版信息

Pharmacogn Mag. 2014 Jul;10(39):254-64. doi: 10.4103/0973-1296.137365.

DOI:10.4103/0973-1296.137365
PMID:25210312
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4159918/
Abstract

BACKGROUND

Danmu injection, a traditional Chinese medicine (TCM) preparation made from Nauclea officinalis, has been commonly used for the treatment of cold, fever, swelling of throat in China. However, the chemical constituents in Danmu injection have not been clarified yet.

OBJECTIVE

a HPLC/DAD/ESI-MS(n) method was developed for qualitative and quantitative analysis of the components in Danmu injection.

MATERIALS AND METHODS

The chromatographic separation was performed on a Welch Material XB-C18 (4.6mm × 250mm, 5μm) using gradient elution with acetonitrile (A) and water containing 0.1% formic acid (B) as mobile phase at a flow rate of 1.0 ml/min.

RESULTS

Twenty-five compounds, including phenolic acid and phenol glycoside, iridous glycoside and glycoalkaloid were identified or tentatively deduced on the base of their retention behaviors, UV absorption, MS and MS(n) data with those elucidated references or literature. In addition, eleven compounds were simultaneously determined by HPLC-DAD, which was validated and successfully applied for determination of major components in Danmu injection.

CONCLUSION

The results suggested that the established qualitative and quantitative method would be a powerful and reliable analytical tool for the characterization of multi-constituent in complex chemical system and quality control of Danmu injection.

摘要

背景

丹木注射液是一种由乌檀制成的中药制剂，在中国常用于治疗感冒、发热、咽喉肿痛。然而，丹木注射液中的化学成分尚未明确。

目的

建立一种HPLC/DAD/ESI-MS(n)方法用于丹木注射液中成分的定性和定量分析。

材料与方法

采用Welch Material XB-C18（4.6mm×250mm，5μm）色谱柱，以乙腈（A）和含0.1%甲酸的水（B）为流动相进行梯度洗脱，流速为1.0ml/min。

结果

根据保留行为、紫外吸收、质谱和串联质谱数据，与已阐明的参考文献或文献对照，鉴定或初步推断出25种化合物，包括酚酸和酚苷、环烯醚萜苷和生物碱苷。此外，采用HPLC-DAD同时测定了11种化合物，该方法经过验证并成功应用于丹木注射液中主要成分的测定。

结论

结果表明，所建立的定性和定量方法将是表征复杂化学体系中多成分以及丹木注射液质量控制的有力且可靠的分析工具。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/97ef/4159918/f356a9fd56f4/PM-10-254-g012.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/97ef/4159918/20a996c121d0/PM-10-254-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/97ef/4159918/1c3ad84383b0/PM-10-254-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/97ef/4159918/d0d24584d80a/PM-10-254-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/97ef/4159918/695012a6b56a/PM-10-254-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/97ef/4159918/c512e4a18ff6/PM-10-254-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/97ef/4159918/6bb4d6073fee/PM-10-254-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/97ef/4159918/cf3dec447452/PM-10-254-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/97ef/4159918/4560ac5ca32e/PM-10-254-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/97ef/4159918/6f2ef716dbb2/PM-10-254-g011.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/97ef/4159918/f356a9fd56f4/PM-10-254-g012.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/97ef/4159918/20a996c121d0/PM-10-254-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/97ef/4159918/1c3ad84383b0/PM-10-254-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/97ef/4159918/d0d24584d80a/PM-10-254-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/97ef/4159918/695012a6b56a/PM-10-254-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/97ef/4159918/c512e4a18ff6/PM-10-254-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/97ef/4159918/6bb4d6073fee/PM-10-254-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/97ef/4159918/cf3dec447452/PM-10-254-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/97ef/4159918/4560ac5ca32e/PM-10-254-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/97ef/4159918/6f2ef716dbb2/PM-10-254-g011.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/97ef/4159918/f356a9fd56f4/PM-10-254-g012.jpg

相似文献

1
Qualitative and quantitative analysis of the major constituents in traditional Chinese medicine Danmu injection using LC-ESI-MS(n) and LC-DAD.采用液相色谱-电喷雾串联质谱法（LC-ESI-MS(n)）和液相色谱-二极管阵列检测法（LC-DAD）对中药丹木注射液中的主要成分进行定性和定量分析。
Pharmacogn Mag. 2014 Jul;10(39):254-64. doi: 10.4103/0973-1296.137365.
2
Chromatographic fingerprints analysis and determination of seven components in Danmu preparations by HPLC-DAD/QTOF-MS.丹木制剂中七种成分的HPLC-DAD/QTOF-MS色谱指纹图谱分析与测定
Chin Med. 2020 Feb 18;15:19. doi: 10.1186/s13020-020-00301-5. eCollection 2020.
3
Simultaneous determination of six alkaloid components in rat plasma and its application to pharmacokinetic study of Danmu preparations by an ultra fast liquid chromatography-electrospray ionization-tandem mass spectrometry.超快速液相色谱-电喷雾电离-串联质谱法同时测定大鼠血浆中六种生物碱成分及其在丹木制剂药代动力学研究中的应用
J Chromatogr B Analyt Technol Biomed Life Sci. 2015 Mar 1;983-984:10-7. doi: 10.1016/j.jchromb.2014.12.026. Epub 2015 Jan 3.
4
Qualitative and quantitative analysis of the constituents in Danmu preparations by UPLC-PDA-TOF-MS.超高效液相色谱-光电二极管阵列-飞行时间质谱法对丹木制剂成分的定性和定量分析
J Chromatogr Sci. 2014 Sep;52(8):862-71. doi: 10.1093/chromsci/bmt129. Epub 2013 Aug 28.
5
Qualitative and quantitative analysis of the major constituents in Chinese medicinal preparation Guan-Xin-Ning injection by HPLC-DAD-ESI-MS(n).采用高效液相色谱-二极管阵列检测-电喷雾串联质谱联用技术对中药制剂冠心宁注射液中主要成分进行定性和定量分析。
J Pharm Biomed Anal. 2012 Feb 5;59:184-9. doi: 10.1016/j.jpba.2011.10.007. Epub 2011 Oct 15.
6
Identification and determination of the major constituents in Traditional Chinese Medicinal formula Danggui-Shaoyao-San by HPLC-DAD-ESI-MS/MS.采用高效液相色谱-二极管阵列检测器-电喷雾串联质谱法鉴定和测定中药方剂当归芍药散中的主要成分。
J Pharm Biomed Anal. 2009 Sep 8;50(2):127-37. doi: 10.1016/j.jpba.2009.03.039. Epub 2009 Apr 8.
7
HPLC-DAD-MS(n) analysis and HPLC quantitation of chemical constituents in Xian-ling-gu-bao capsules.HPLC-DAD-MS(n) 分析及 HPLC 定量法测定仙鹿口服液中的化学成分。
J Pharm Biomed Anal. 2011 Jul 15;55(5):923-33. doi: 10.1016/j.jpba.2011.03.021. Epub 2011 Mar 21.
8
Identification and Simultaneous Determination of Twelve Active Components in the Methanol Extract of Traditional Medicine Weichang'an Pill by HPLC-DAD-ESI-MS/MS.高效液相色谱-二极管阵列检测-电喷雾串联质谱法鉴定并同时测定传统药物胃肠安丸甲醇提取物中的12种活性成分
Iran J Pharm Res. 2013 Winter;12(1):15-24.
9
Identification and determination of the major constituents in traditional Chinese medicine Si-Wu-Tang by HPLC coupled with DAD and ESI-MS.高效液相色谱-二极管阵列检测-电喷雾电离质谱联用技术鉴定与测定中药四物汤中的主要成分
J Pharm Biomed Anal. 2004 Feb 18;34(3):705-13. doi: 10.1016/s0731-7085(03)00650-2.
10
Qualitative and quantitative characterization of chemical constituents in Xin-Ke-Shu preparations by liquid chromatography coupled with a LTQ Orbitrap mass spectrometer.采用液相色谱-线性离子阱轨道阱质谱联用技术对心可舒制剂中的化学成分进行定性和定量分析。
J Pharm Biomed Anal. 2011 Jul 15;55(5):984-95. doi: 10.1016/j.jpba.2011.03.045. Epub 2011 Apr 7.

引用本文的文献

1
Phytochemical Analysis and Antidiarrheal Activity of Stem Bark Decoctions of Sabine (Clusiaceae).西番莲（藤黄科）茎皮煎剂的植物化学分析及止泻活性
Molecules. 2024 Dec 7;29(23):5789. doi: 10.3390/molecules29235789.
2
The Applications and Features of Liquid Chromatography-Mass Spectrometry in the Analysis of Traditional Chinese Medicine.液相色谱-质谱联用技术在中药分析中的应用及特点
Evid Based Complement Alternat Med. 2016;2016:3837270. doi: 10.1155/2016/3837270. Epub 2016 Nov 10.

本文引用的文献

1
[Chemical constituents of Nauclea officinalis].[乌檀的化学成分]
Yao Xue Xue Bao. 2013 Feb;48(2):276-80.
2
Qualitative and quantitative analysis of the major constituents in Chinese medicinal preparation Guan-Xin-Ning injection by HPLC-DAD-ESI-MS(n).采用高效液相色谱-二极管阵列检测-电喷雾串联质谱联用技术对中药制剂冠心宁注射液中主要成分进行定性和定量分析。
J Pharm Biomed Anal. 2012 Feb 5;59:184-9. doi: 10.1016/j.jpba.2011.10.007. Epub 2011 Oct 15.
3
[Improvement and enhancement of traditional Chinese medicine injections].[中药注射剂的改进与提升]
Zhongguo Zhong Yao Za Zhi. 2011 Jul;36(14):1905-9.
4
Identification of indole alkaloids in Nauclea officinalis using high-performance liquid chromatography coupled with ion trap and time-of-flight mass spectrometry.使用高效液相色谱-离子阱和飞行时间质谱联用技术鉴定乌檀中的吲哚生物碱
Eur J Mass Spectrom (Chichester). 2011;17(3):277-86. doi: 10.1255/ejms.1121.
5
Recent advances on HPLC/MS in medicinal plant analysis.高效液相色谱/质谱联用技术在药用植物分析中的最新进展。
J Pharm Biomed Anal. 2011 Jun 25;55(4):744-57. doi: 10.1016/j.jpba.2010.11.015. Epub 2010 Nov 19.
6
Characterisation of commercially available linear alkylbenzenesulfonates by LC-SPE-NMR/MS (liquid chromatography-solid phase extraction-nuclear magnetic resonance spectroscopy-mass spectroscopy).采用 LC-SPE-NMR/MS（液相色谱-固相萃取-核磁共振波谱-质谱联用）对市售直链烷基苯磺酸盐进行表征。
Talanta. 2010 Jun 30;82(1):143-50. doi: 10.1016/j.talanta.2010.04.011. Epub 2010 Apr 18.
7
Rapid analysis of a Chinese herbal prescription by liquid chromatography-time-of-flight tandem mass spectrometry.采用液相色谱-飞行时间串联质谱法快速分析中药复方
J Chromatogr A. 2008 Oct 10;1206(2):140-6. doi: 10.1016/j.chroma.2008.08.038. Epub 2008 Aug 15.
8
[Determination of the allergic impurities in the parenteral injection of Chinese traditional medicines containing Salvia miltiorrhiza].[含丹参的中药注射剂中过敏性杂质的测定]
Yao Xue Xue Bao. 2008 May;43(5):518-22.
9
Hypothesis of active components in volatile oil from a Chinese herb formulation, 'Shao-Fu-Zhu-Yu decoction', using GC-MS and chemometrics.运用气相色谱-质谱联用技术（GC-MS）和化学计量学对中药方剂“少腹逐瘀汤”挥发油中的活性成分进行研究的假设。
J Sep Sci. 2008 Apr;31(6-7):1085-91. doi: 10.1002/jssc.200700492.
10
A rapid method for the simultaneous determination of 11 saponins in Panax notoginseng using ultra performance liquid chromatography.一种使用超高效液相色谱法同时测定三七中11种皂苷的快速方法。
J Pharm Biomed Anal. 2007 Aug 15;44(4):996-1000. doi: 10.1016/j.jpba.2007.03.032. Epub 2007 Apr 4.