Marks Michael, Goncalves Adriana, Vahi Ventis, Sokana Oliver, Puiahi Elliot, Zhang Zaixing, Dalipanda Tenneth, Bottomley Christian, Mabey David, Solomon Anthony W
Clinical Research Department, Faculty of Tropical and Infectious Diseases, London School of Hygiene & Tropical Medicine, London, United Kingdom; Hospital for Tropical Diseases, University College London Hospitals NHS Trust, Mortimer Market, London, United Kingdom.
Clinical Research Department, Faculty of Tropical and Infectious Diseases, London School of Hygiene & Tropical Medicine, London, United Kingdom.
PLoS Negl Trop Dis. 2014 Sep 11;8(9):e3156. doi: 10.1371/journal.pntd.0003156. eCollection 2014 Sep.
Yaws is a non-venereal treponemal infection caused by Treponema pallidum ssp. pertenue. The WHO has launched a worldwide control programme, which aims to eradicate yaws by 2020. The development of a rapid diagnostic test (RDT) for serological diagnosis in the isolated communities affected by yaws is a key requirement for the successful implementation of the WHO strategy. We conducted a study to evaluate the utility of the DPP test in screening for yaws, utilizing samples collected as part of a community prevalence survey conducted in the Solomon Islands. 415 serum samples were tested using both traditional syphilis serology (TPPA and quantitative RPR) and the Chembio DPP Syphilis Screen and Confirm RDT. We calculated the sensitivity and specificity of the RDT as compared to gold standard serology. The sensitivity of the RDT against TPPA was 58.5% and the specificity was 97.6%. The sensitivity of the RDT against RPR was 41.7% and the specificity was 95.2%. The sensitivity of the DPP was strongly related to the RPR titre with a sensitivity of 92.0% for an RPR titre of >1/16. Wider access to DPP testing would improve our understanding of worldwide yaws case reporting and the test may play a key role in assessing patients presenting with yaws like lesions in a post-mass drug administration (MDA) setting.
雅司病是由苍白密螺旋体地方亚种引起的一种非性病性螺旋体感染。世界卫生组织已启动一项全球防治计划，目标是到2020年根除雅司病。开发一种用于受雅司病影响的孤立社区血清学诊断的快速诊断检测（RDT），是成功实施世卫组织战略的一项关键要求。我们开展了一项研究，利用在所罗门群岛进行的社区患病率调查所收集的样本，评估双抗原夹心免疫层析法（DPP）检测在筛查雅司病中的效用。使用传统梅毒血清学检测方法（梅毒螺旋体颗粒凝集试验和定量快速血浆反应素环状卡片试验）以及Chembio DPP梅毒筛查和确认RDT对415份血清样本进行了检测。我们计算了该RDT与金标准血清学检测相比的灵敏度和特异性。该RDT相对于梅毒螺旋体颗粒凝集试验的灵敏度为58.5%，特异性为97.6%。该RDT相对于快速血浆反应素环状卡片试验的灵敏度为41.7%，特异性为95.2%。双抗原夹心免疫层析法的灵敏度与快速血浆反应素环状卡片试验滴度密切相关，对于滴度>1/16的样本，灵敏度为92.0%。更广泛地使用双抗原夹心免疫层析法检测将增进我们对全球雅司病病例报告的了解，并且该检测可能在评估在大规模药物给药（MDA）后出现类似雅司病病变的患者时发挥关键作用。
