Chen Sihan, Huang Siyuan, Son Jangyup, Han Edmund, Watanabe Kenji, Taniguchi Takashi, Huang Pinshane Y, King William P, van der Zande Arend M, Bashir Rashid
Holonyak Micro and Nanotechnology Laboratory, University of Illinois Urbana-Champaign, Urbana, IL 61801, United States.
Department of Mechanical Science and Engineering, University of Illinois Urbana-Champaign, Urbana, IL 61801, United States.
Res Sq. 2024 Nov 11:rs.3.rs-5193820. doi: 10.21203/rs.3.rs-5193820/v2.
A long-unrealized goal in solid-state nanopore sensing is to achieve out-of-plane electrical sensing and control of DNA during translocation, which is a prerequisite for base-by-base ratcheting that enables DNA sequencing in biological nanopores. Two-dimensional (2D) heterostructures, with their capability to construct out-of-plane electronics with atomic layer precision, are ideal yet unexplored candidates for use as electrical sensing membranes. Here we demonstrate a nanopore architecture using a vertical 2D heterojunction diode consisting of p-type WSe on n-type MoS. This diode exhibits rectified interlayer tunneling currents modulated by ionic potential, while the heterojunction potential reciprocally rectifies ionic transport through the nanopore. We achieve concurrent detection of DNA translocation using both ionic and diode currents and demonstrate a 2.3-fold electrostatic slowing of translocation speed. Encapsulation layers enable robust operation while preserving the spatial resolution of atomically sharp 2D heterointerface for sensing. These results establish a paradigm for out-of-plane electrical sensing and control of single biomolecules.
固态纳米孔传感中一个长期未实现的目标是在DNA易位过程中实现平面外电传感和控制，这是逐碱基棘轮效应的先决条件，而逐碱基棘轮效应能够在生物纳米孔中进行DNA测序。二维（2D）异质结构能够以原子层精度构建平面外电子器件，是用作电传感膜的理想但尚未探索的候选材料。在此，我们展示了一种纳米孔结构，其使用由n型MoS上的p型WSe组成的垂直2D异质结二极管。该二极管表现出由离子势调制的整流层间隧穿电流，而异质结势则反向整流通过纳米孔的离子传输。我们利用离子电流和二极管电流同时实现了对DNA易位的检测，并证明易位速度有2.3倍的静电减慢。封装层能够实现稳健操作，同时保留用于传感的原子级尖锐2D异质界面的空间分辨率。这些结果建立了一种用于单生物分子平面外电传感和控制的范例。
