Bek-Thomsen Malene, Lomholt Hans B, Scavenius Carsten, Enghild Jan J, Brüggemann Holger
Department of Biomedicine, Aarhus University, Aarhus, Denmark.
Department of Molecular Biology and Genetics, Aarhus University, Aarhus, Denmark.
PLoS One. 2014 Sep 19;9(9):e107908. doi: 10.1371/journal.pone.0107908. eCollection 2014.
Acne vulgaris is a very common disease of the pilosebaceous unit of the human skin. The pathological processes of acne are not fully understood. To gain further insight sebaceous follicular casts were extracted from 18 healthy and 20 acne-affected individuals by cyanoacrylate-gel biopsies and further processed for mass spectrometry analysis, aiming at a proteomic analysis of the sebaceous follicular casts. Human as well as bacterial proteins were identified. Human proteins enriched in acne and normal samples were detected, respectively. Normal follicular casts are enriched in proteins such as prohibitins and peroxiredoxins which are involved in the protection from various stresses, including reactive oxygen species. By contrast, follicular casts extracted from acne-affected skin contained proteins involved in inflammation, wound healing and tissue remodeling. Among the most distinguishing proteins were myeloperoxidase, lactotransferrin, neutrophil elastase inhibitor and surprisingly, vimentin. The most significant biological process among all acne-enriched proteins was 'response to a bacterium'. Identified bacterial proteins were exclusively from Propionibacterium acnes. The most abundant P. acnes proteins were surface-exposed dermatan sulphate adhesins, CAMP factors, and a so far uncharacterized lipase in follicular casts extracted from normal as well as acne-affected skin. This is a first proteomic study that identified human proteins together with proteins of the skin microbiota in sebaceous follicular casts.
寻常痤疮是一种常见的人类皮肤毛囊皮脂腺疾病。痤疮的病理过程尚未完全明了。为了深入了解，通过氰基丙烯酸酯凝胶活检从18名健康个体和20名痤疮患者中提取了皮脂腺毛囊管型，并进一步进行质谱分析，旨在对皮脂腺毛囊管型进行蛋白质组学分析。鉴定出了人类和细菌蛋白。分别检测了痤疮样本和正常样本中富集的人类蛋白。正常毛囊管型富含如抑癌蛋白和过氧化物酶等参与抵御包括活性氧在内的各种应激的蛋白质。相比之下，从痤疮皮肤中提取的毛囊管型含有参与炎症、伤口愈合和组织重塑的蛋白质。其中最具特征性的蛋白质是髓过氧化物酶、乳铁传递蛋白、中性粒细胞弹性蛋白酶抑制剂，令人惊讶的是还有波形蛋白。所有痤疮富集蛋白中最显著的生物学过程是“对细菌的反应”。鉴定出的细菌蛋白仅来自痤疮丙酸杆菌。在从正常皮肤和痤疮皮肤中提取的毛囊管型中，痤疮丙酸杆菌最丰富的蛋白质是表面暴露的硫酸皮肤素粘附素、CAMP因子和一种迄今未鉴定的脂肪酶。这是第一项在皮脂腺毛囊管型中鉴定人类蛋白以及皮肤微生物群蛋白的蛋白质组学研究。
