口腔冲洗液样本中的新型DNA甲基化靶点可预测口腔鳞状细胞癌患者的生存率。
Novel DNA methylation targets in oral rinse samples predict survival of patients with oral squamous cell carcinoma.
作者信息
Langevin Scott M, Butler Rondi A, Eliot Melissa, Pawlita Michael, Maccani Jennifer Z J, McClean Michael D, Kelsey Karl T
机构信息
Department of Environmental Health, University of Cincinnati College of Medicine, Cincinnati, OH, USA.
Department of Epidemiology, Brown University, Providence, RI, USA.
出版信息
Oral Oncol. 2014 Nov;50(11):1072-80. doi: 10.1016/j.oraloncology.2014.08.015. Epub 2014 Sep 18.
OBJECTIVES
The objective of this study was to identify novel survival-associated biomarkers in oral rinse samples collected from patients with oral squamous cell carcinoma (OSCC).
MATERIALS AND METHODS
We screened for putative survival-associated markers using publicly available methylation array data from 88 OSCC tumors. Cox models were then fit to methylation array data restricted to these putative loci in oral rinse samples of 82 OSCC patients from greater Boston. Pyrosequencing assays were designed for each locus that replicated in the oral rinse samples and applied to a validation set of oral rinse samples from another 61 OSCC patients.
RESULTS
We identified 7 survival-associated methylation markers in oral rinse samples from OSCC patients, and have validated one, located in the body of GABBR1, by pyrosequencing.
CONCLUSION
The 7 CpG loci identified through this study represent novel prognostic biomarkers for patients with OSCC that can be detected using a non-invasive oral rinse collection technique.
目的
本研究的目的是在从口腔鳞状细胞癌（OSCC）患者收集的漱口液样本中鉴定新的生存相关生物标志物。
材料与方法
我们使用来自88例OSCC肿瘤的公开甲基化阵列数据筛选假定的生存相关标志物。然后将Cox模型应用于来自大波士顿地区82例OSCC患者漱口液样本中这些假定位点的甲基化阵列数据。针对在漱口液样本中重复出现的每个位点设计焦磷酸测序分析，并应用于来自另外61例OSCC患者的漱口液样本验证集。
结果
我们在OSCC患者的漱口液样本中鉴定出7个生存相关甲基化标志物，并通过焦磷酸测序验证了位于GABBR1基因体中的1个标志物。
结论
通过本研究鉴定出的7个CpG位点代表了OSCC患者新的预后生物标志物，可使用非侵入性漱口液收集技术进行检测。
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