Cardoso Cristiane de Almeida Baldini, Lacerda Bianca, Mangueira Dayane Franco Barros, Charone Senda, Olympio Kelly Polido Kaneshiro, Magalhães Ana Carolina, Pessan Juliano Pelim, Vilhena Fabiano Vieira, Sampaio Fábio Correia, Buzalaf Marília Afonso Rabelo
Department of Biological Sciences, Bauru Dental School, University of São Paulo, Al. Octávio Pinheiro Brisolla, 9-75, Bauru, SP 17012-901, Brazil.
Department of Clinical and Social Dentistry, Paraíba Federal University, Jardim Universitário, s/n, João Pessoa, PB 58051900, Brazil.
Arch Oral Biol. 2015 Jan;60(1):23-8. doi: 10.1016/j.archoralbio.2014.08.016. Epub 2014 Sep 6.
This study attempted to clarify the mechanisms of action of fluoridated acidic liquid dentifrices against dental caries.
In the in vitro leg, enamel specimens were submitted to a pH-cycling model, treated with distinct dentifrices (0, 550 μgF/g pH 4.5 and pH 7.0, 1100 or 5000 μgF/g pH 7.0) and analyzed using hardness. Alkali-soluble fluoride (F) deposition was quantified on pre-demineralized specimens treated with the dentifrices. In the clinical leg, 2-to-4-year-old children who had been using liquid dentifrices for 6 months (550 μgF/g pH 4.5 or pH 7.0 or 1100 μgF/g pH 7.0) had their plaque samples collected 5 and 60 min after the last brushing. Fluoride uptake in whole plaque was evaluated.
The reduction of the pH had a partial preventive effect on subsurface hardness loss only. [F] had a significant influence on the deposition of fluoride, surface and subsurface hardness loss. In vivo, the reduction of the pH was able to significantly increase plaque F uptake, leading to similar levels as those found for the neutral dentifrice containing twice [F].
The results obtained from in vitro studies whose design does not include the presence of dental plaque should be interpreted with caution.
本研究旨在阐明含氟酸性液体牙膏预防龋齿的作用机制。
在体外实验中，将釉质标本置于pH循环模型中，用不同的牙膏（0、550μgF/g pH 4.5和pH 7.0、1100或5000μgF/g pH 7.0）处理，并用硬度分析。对用牙膏处理过的预脱矿标本上的碱溶性氟（F）沉积进行定量分析。在临床实验中，收集使用液体牙膏6个月（550μgF/g pH 4.5或pH 7.0或1100μgF/g pH 7.0）的2至4岁儿童在最后一次刷牙后5分钟和60分钟的牙菌斑样本。评估整个牙菌斑中的氟摄取情况。
pH值降低仅对表面下硬度损失有部分预防作用。[F]对氟沉积、表面和表面下硬度损失有显著影响。在体内，pH值降低能够显著增加牙菌斑对氟的摄取，使其达到含两倍[F]的中性牙膏的水平。
对于设计中未包括牙菌斑存在情况的体外研究结果，应谨慎解读。
