霍奇金淋巴瘤中P16蛋白表达缺失及其与爱泼斯坦-巴尔病毒LMP-1表达的关系

Loss of P16 Protein Expression and Its Association with Epstein-Barr Virus LMP-1 Expression in Hodgkin's Lymphoma.

作者信息

Irshaid Fawzi, Tarawneh Khaled, Alshdefat Aisha, Dilmi Fatiha, Jaran Adnan, Al-Hadithi Raji, Al-Khatib Ahad

机构信息

Dept. of Biological Sciences, Faculty of Science, Al al-Bayt University, Al-Mafraq, Jordan.

出版信息

Iran J Cancer Prev. 2013 Spring;6(2):78-84.

PMID:25250115

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4142916/

Abstract

BACKGROUND

Expression of Epstein-Barr virus Latent Member Protein-1 (EBV LMP-1) and loss of P16 protein expression are documented in lymphoma, indicating a relationship between them, but this relationship is not clear and sometimes contradictory. Thus, this study was conducted to examine the relationship between the loss of P16 and EBV LMP-1 expression in Jordanian patients diagnosed with lymphoma.

METHODS

Sections were made from archival formalin-fixed and paraffin-embedded blocks from 55 patients diagnosed with lymphoma. P16 expression and LMP-1 expression were detected by immunohistochemistry using monoclonal antibodies.

RESULTS

In Hodgkin's Lymphoma (HL), the loss of P16 was higher in LMP-1 positive cases (61%) than LMP-1 negative cases (25%; P = 0.072). Conversely, in Non-Hodgkin's Lymphoma (NHL), none of LMP-1 positive samples showed loss of P16. Furthermore, among LMP-1 HL positive cases, the loss of P16 was more frequent in male (75%) than female (33%). Also, there was a significantly higher proportion of LMP-1 positive cases showing loss of P16 in HL (11:18), compared to those in NHL (0:8, P < 0.001), confirming a difference between HL and NHL, concerning the LMP-1/P16 relationship.

CONCLUSION

A trend for an association between loss of P16 and LMP-1 expression was observed in HL but not NHL patients. These findings suggest that there are molecular and clinical differences in the pathogenesis and development of different subtypes of lymphoma.

摘要

背景

在淋巴瘤中已证实存在爱泼斯坦-巴尔病毒潜伏膜蛋白1（EBV LMP-1）的表达以及P16蛋白表达缺失，表明二者之间存在关联，但这种关系尚不明确，且有时相互矛盾。因此，本研究旨在探讨约旦淋巴瘤患者中P16缺失与EBV LMP-1表达之间的关系。

方法

从55例淋巴瘤确诊患者的福尔马林固定石蜡包埋存档组织块中制备切片。使用单克隆抗体通过免疫组织化学检测P16表达和LMP-1表达。

结果

在霍奇金淋巴瘤（HL）中，LMP-1阳性病例的P16缺失率（61%）高于LMP-1阴性病例（25%；P = 0.072）。相反，在非霍奇金淋巴瘤（NHL）中，LMP-1阳性样本均未显示P16缺失。此外，在LMP-1阳性的HL病例中，男性的P16缺失更为常见（75%），而女性为（33%）。而且，HL中显示P16缺失的LMP-1阳性病例比例（11:18）显著高于NHL（0:8，P < 0.001），证实了HL和NHL在LMP-1/P16关系方面存在差异。