Artigas C, Navea A, López-Murcia M-M, Felipe A, Desco C, Artigas J-M
Facultad de Veterinaria, Universidad Cardenal Herrera-CEU, Avda Seminario s/n, 46113 Moncada (Valencia), Spain.
Fisabio Oftalmología Médica (FOM), Bifurcación Pío Baroja-General Avilés, s/n, 46015 Valencia, Spain.
J Fr Ophtalmol. 2014 Dec;37(10):773-9. doi: 10.1016/j.jfo.2014.06.006. Epub 2014 Oct 3.
To determine the spectral transmission curve of the crystalline lens of the pig. To analyse how this curve changes when the crystalline lens is irradiated with ultraviolet A+B radiation similar to that of the sun. To compare these results with literature data from the human crystalline lens.
We used crystalline lenses of the common pig from a slaughterhouse, i.e. genetically similar pigs, fed with the same diet, and slaughtered at six months old. Spectral transmission was measured with a Perkin-Elmer Lambda 35 UV/VIS spectrometer. The lenses were irradiated using an Asahi Spectra Lax-C100 ultraviolet source, which made it possible to select the spectral emission band as well as the intensity and exposure time.
The pig lens transmits all the visible spectrum (95%) and lets part of the ultraviolet A through (15%). Exposure to acute UV (A+B) irradiation causes a decrease in its transmission as the intensity or exposure time increases: this decrease is considerable in the UV region.
We were able to determine the mean spectral transmission curve of the pig lens. It appears to be similar to that of the human lens in the visible spectrum, but different in the ultraviolet. Pig lens transmission is reduced by UV (A+B) irradiation and its transmission in the UV region can even disappear as the intensity or exposure time increases. An adequate exposure intensity and time of UV (A+B) radiation always causes an anterior subcapsular cataract (ASC).
确定猪晶状体的光谱透射曲线。分析当晶状体受到类似于太阳光的紫外线A+B辐射照射时,该曲线如何变化。将这些结果与人类晶状体的文献数据进行比较。
我们使用了来自屠宰场的普通猪的晶状体,即基因相似、食用相同饲料且6个月大时宰杀的猪的晶状体。使用珀金埃尔默Lambda 35紫外/可见光谱仪测量光谱透射率。使用朝日光谱Lax-C100紫外光源对晶状体进行照射,该光源可以选择光谱发射带以及强度和曝光时间。
猪晶状体透射所有可见光谱(95%),并让部分紫外线A通过(15%)。暴露于急性紫外线(A+B)照射会导致其透射率随着强度或曝光时间的增加而降低:在紫外线区域这种降低相当显著。
我们能够确定猪晶状体的平均光谱透射曲线。在可见光谱中它似乎与人类晶状体的相似,但在紫外线区域不同。猪晶状体的透射率会因紫外线(A+B)照射而降低,并且随着强度或曝光时间的增加,其在紫外线区域的透射率甚至可能消失。适当的紫外线(A+B)辐射暴露强度和时间总是会导致前囊下白内障(ASC)。