Tanzi Elisabetta, Bianchi Silvia, Frati Elena R, Amicizia Daniela, Martinelli Marianna, Bragazzi Nicola L, Brisigotti Maria Pia, Colzani Daniela, Fasoli Ester, Zehender Gianguglielmo, Panatto Donatella, Gasparini Roberto
Department of Biomedical Sciences for Health, University of Milan, Milan, Italy.
Department of Health Sciences, University of Genoa, Genoa, Italy.
J Gen Virol. 2015 Jan;96(Pt 1):206-209. doi: 10.1099/vir.0.070672-0. Epub 2014 Oct 8.
Human papillomavirus (HPV) has a well-recognized aetiological role in the development of cervical cancer and other anogenital tumours. Recently, an association between colorectal cancer and HPV infection has been suggested, although this is still controversial. This study aimed at detecting and characterizing HPV infection in 57 paired biopsies from colorectal cancers and adjacent intact tissues using a degenerate PCR approach. All amplified fragments were genotyped by means of sequencing. Overall, HPV prevalence was 12.3 %. In particular, 15.8 % of tumour tissues and 8.8 % of non-cancerous tissue samples were HPV DNA-positive. Of these samples, 85.7 % were genotyped successfully, with 41.7 % of sequences identifying four genotypes of the HR (high oncogenic risk) clade Group 1; the remaining 58.3 % of HPV-genotyped specimens had an unclassified β-HPV. Examining additional cases and analysing whole genomes will help to outline the significance of these findings.
人乳头瘤病毒（HPV）在宫颈癌和其他肛门生殖器肿瘤的发生发展中具有公认的病因学作用。最近，有人提出结直肠癌与HPV感染之间存在关联，尽管这一观点仍存在争议。本研究旨在采用简并PCR方法检测并鉴定57对来自结直肠癌及其相邻完整组织的活检样本中的HPV感染情况。所有扩增片段均通过测序进行基因分型。总体而言，HPV感染率为12.3%。具体来说，15.8%的肿瘤组织和8.8%的非癌组织样本HPV DNA呈阳性。在这些样本中，85.7%成功进行了基因分型，其中41.7%的序列鉴定出HR（高致癌风险）进化枝1组的四种基因型；其余58.3%的HPV基因分型标本具有未分类的β-HPV。检查更多病例并分析全基因组将有助于阐明这些发现的意义。
