Yang Mao, Fu Zhenkun, Zhang Qingjiang, Xin Yu, Chen Yanjun, Tian Ye
Department of Cardiology, The Fourth Affiliated Hospital of Harbin Medical University, Harbin, China.
Department of Immunology, Harbin Medical University, Harbin, China.
PLoS One. 2014 Oct 13;9(10):e109658. doi: 10.1371/journal.pone.0109658. eCollection 2014.
Intercellular adhesion molecule-1 (ICAM-1), an important immune adhesion molecule, is related to the atherosclerosis. We explored the association between the polymorphisms of the ICAM-1 gene and coronary atherosclerotic stenosis to determine whether any risk factors correlate with genetic polymorphisms in Chinese patients with coronary atherosclerosis. Using the SNaPshot assay, we examined six SNPs of rs5491, rs281428, rs281432, rs5496, rs5498 and rs281437 in 604 patients diagnosed with coronary atherosclerotic stenosis by angiography and in 468 controls. We found that AG genotype of rs5498 had higher frequency in the coronary atherosclerotic stenosis patients (41.56% to 34.19%, P = 0.017, OR = 1.368,95%CI 1.057-1.770) and that the haplotype Ars5491Crs281428Grs281432 had higher frequency in patients (13.8% to 12.1%, P = 0.048). When analyzing the clinical risk factors for coronary atherosclerosis, we found that the rs5498 locus was associated with the levels of apolipoprotein A (APOA) (P = 0.0002) and triglycerides (TG) (P = 0.002). Furthermore, the levels of triglycerides (TG) were also associated with rs281432 (P = 0.040). Additionally, the TT genotype of rs281437 was associated with a higher level of apolipoprotein A (APOA) (P = 0.039) and apolipoprotein B (APOB) (P = 0.003). Finally, among those with coronary atherosclerosis, we found no differences in the haplotype analysis of polymorphisms of the ICAM-1 gene from individuals with hypertension or those who smoked. According to our results, the ICAM-1 polymorphisms were associated with risk of coronary atherosclerotic stenosis in Chinese individuals.
细胞间黏附分子-1（ICAM-1）是一种重要的免疫黏附分子，与动脉粥样硬化有关。我们探讨了ICAM-1基因多态性与冠状动脉粥样硬化狭窄之间的关联，以确定在中国冠状动脉粥样硬化患者中是否有任何危险因素与基因多态性相关。我们使用SNaPshot分析法，检测了604例经血管造影诊断为冠状动脉粥样硬化狭窄的患者以及468例对照者中rs5491、rs281428、rs281432、rs5496、rs5498和rs281437这6个单核苷酸多态性（SNP）。我们发现，rs5498的AG基因型在冠状动脉粥样硬化狭窄患者中的频率较高（41.56%对34.19%，P = 0.017，比值比[OR]=1.368，95%可信区间[CI]为1.057 - 1.770），并且单倍型Ars5491Crs281428Grs281432在患者中的频率较高（13.8%对12.1%，P = 0.048）。在分析冠状动脉粥样硬化的临床危险因素时，我们发现rs5498位点与载脂蛋白A（APOA）水平（P = 0.0002）和甘油三酯（TG）水平（P = 0.002）相关。此外，甘油三酯（TG）水平也与rs281432相关（P = 0.040）。另外，rs281437的TT基因型与较高的载脂蛋白A（APOA）水平（P = 0.039）和载脂蛋白B（APOB）水平（P = 0.003）相关。最后，在冠状动脉粥样硬化患者中，我们发现高血压患者或吸烟者的ICAM-1基因多态性单倍型分析没有差异。根据我们的结果，ICAM-1基因多态性与中国人群冠状动脉粥样硬化狭窄风险相关。
