川西獐牙菜甲醇提取物的抗氧化和抗菌活性评价
Evaluation of antioxidant and antibacterial activity of methanolic extracts of Gentiana kurroo royle.
作者信息
Baba Shoib A, Malik Shahid A
机构信息
Department of Botany, University of Kashmir, Srinagar 190001, Jammu and Kashmir, India.
Department of Chemistry, University of Kashmir, Srinagar 190001, Jammu and Kashmir, India.
出版信息
Saudi J Biol Sci. 2014 Nov;21(5):493-8. doi: 10.1016/j.sjbs.2014.06.004. Epub 2014 Jun 27.
Abstract
In this study our objective was to evaluate the antioxidant and antimicrobial activity of methanolic extracts of leaves and roots of Gentiana kurroo. The antioxidant activities of the extracts were examined using different biochemical assays namely diphenylpicrylhydrazyl (DPPH), nitroblue tetrazolium (NBT) and ferric reducing power (FRAP). In all the assays, root extract exhibited stronger antioxidant activity than that of leaves. The antibacterial activity of the extracts was also evaluated and MIC values were calculated by broth dilution method. Although, the extracts prevented the growth of both Gram positive and Gram negative bacteria, the MIC values of methanolic extract of the leaves were higher than those of the root extract. The antibacterial and antioxidant activity of the extracts was found to be positively associated with the total phenolic and flavonoid content of the extracts.
摘要
在本研究中，我们的目的是评估藏药秦艽叶和根甲醇提取物的抗氧化和抗菌活性。采用不同的生化分析方法，即二苯基苦味酰基自由基（DPPH）、氮蓝四唑（NBT）和铁还原能力（FRAP）来检测提取物的抗氧化活性。在所有分析中，根提取物表现出比叶提取物更强的抗氧化活性。还评估了提取物的抗菌活性，并通过肉汤稀释法计算了最低抑菌浓度（MIC）值。虽然提取物能抑制革兰氏阳性菌和革兰氏阴性菌的生长，但叶甲醇提取物的MIC值高于根提取物。发现提取物的抗菌和抗氧化活性与提取物中总酚和黄酮含量呈正相关。
相似文献
1
Evaluation of antioxidant and antibacterial activity of methanolic extracts of Gentiana kurroo royle.川西獐牙菜甲醇提取物的抗氧化和抗菌活性评价
Saudi J Biol Sci. 2014 Nov;21(5):493-8. doi: 10.1016/j.sjbs.2014.06.004. Epub 2014 Jun 27.
2
Induction of apoptosis in human pancreatic MiaPaCa-2 cells through the loss of mitochondrial membrane potential (ΔΨm) by Gentiana kurroo root extract and LC-ESI-MS analysis of its principal constituents.通过秦艽根提取物导致人胰腺 MiaPaCa-2 细胞线粒体膜电位（ΔΨm）丧失诱导细胞凋亡及其主要成分的 LC-ESI-MS 分析
Phytomedicine. 2013 Jun 15;20(8-9):723-33. doi: 10.1016/j.phymed.2013.01.011. Epub 2013 Mar 1.
3
Comparative Analysis of Phenolics, Flavonoids, and Antioxidant and Antibacterial Potential of Methanolic, Hexanic and Aqueous Extracts from Adiantum caudatum Leaves.铁线蕨叶甲醇、己烷和水提取物中酚类、黄酮类物质以及抗氧化和抗菌潜力的比较分析
Antioxidants (Basel). 2015 Jun 4;4(2):394-409. doi: 10.3390/antiox4020394.
4
Antioxidant Content, Antioxidant Activity, and Antibacterial Activity of Five Plants from the Commelinaceae Family.鸭跖草科五种植物的抗氧化成分、抗氧化活性及抗菌活性
Antioxidants (Basel). 2014 Nov 17;3(4):758-69. doi: 10.3390/antiox3040758.
5
Total Phenolic Content and Antioxidant and Antibacterial Activities of ..的总酚含量、抗氧化及抗菌活性
Adv Pharmacol Sci. 2019 Jan 2;2019:7428593. doi: 10.1155/2019/7428593. eCollection 2019.
6
Gentiana kurroo Royle attenuates the metabolic aberrations in diabetic rats; Swertiamarin, swertisin and lupeol being the possible bioactive principles.藏药川西獐牙菜可减轻糖尿病大鼠的代谢异常；獐牙菜苦苷、獐牙菜苷和羽扇豆醇可能是其生物活性成分。
J Complement Integr Med. 2017 Apr 29;14(3):/j/jcim.2017.14.issue-3/jcim-2017-0002/jcim-2017-0002.xml. doi: 10.1515/jcim-2017-0002.
7
Antioxidant, antibacterial activity, and phytochemical characterization of Melaleuca cajuputi extract.白千层提取物的抗氧化、抗菌活性及植物化学特征
BMC Complement Altern Med. 2015 Oct 24;15:385. doi: 10.1186/s12906-015-0914-y.
8
Evaluation of antioxidant and antibacterial activities of aqueous, methanolic and alkaloid extracts from Mitragyna speciosa (Rubiaceae family) leaves.评估来自于含羞草科（Rubiaceae 科）植物米口袋（Mitragyna speciosa）的水提物、醇提物和生物碱提取物的抗氧化和抗菌活性。
Molecules. 2009 Oct 9;14(10):3964-74. doi: 10.3390/molecules14103964.
9
In-vitro cytotoxicity in relation to chemotypic diversity in diploid and tetraploid populations of Gentiana kurroo Royle.与二倍体和四倍体甘肃龙胆种群的化学型多样性有关的体外细胞毒性。
J Ethnopharmacol. 2021 Jun 28;274:113966. doi: 10.1016/j.jep.2021.113966. Epub 2021 Feb 26.
10
Methanolic extracts of Withania somnifera leaves, fruits and roots possess antioxidant properties and antibacterial activities.南非醉茄叶、果和根的甲醇提取物具有抗氧化和抗菌活性。
BMC Complement Altern Med. 2012 Oct 7;12:175. doi: 10.1186/1472-6882-12-175.
引用本文的文献
1
Phytochemical Analysis, Isolation, and Characterization of Gentiopicroside from Gentiana kurroo and Cytotoxicity of Biosynthesized Silver Nanoparticles Against HeLa Cells.藏药湿生扁蕾中龙胆苦苷的植物化学分析、分离、表征及生物合成银纳米颗粒对HeLa细胞的细胞毒性
Appl Biochem Biotechnol. 2025 Mar;197(3):1831-1864. doi: 10.1007/s12010-024-05114-y. Epub 2024 Dec 2.
2
Comparative analysis of the antibacterial efficacy and bioactive components of Thuja occidentalis obtained from four different geographical sites.比较分析四个不同产地的西洋杉的抗菌功效和生物活性成分。
Mol Cell Biochem. 2024 Feb;479(2):283-296. doi: 10.1007/s11010-023-04729-9. Epub 2023 Apr 15.
3
Formulation and Characterization of Gum Arabic Stabilized Red Rice Extract Nanoemulsion.阿拉伯胶稳定红米提取物纳米乳液的配方与表征
Polymers (Basel). 2022 May 10;14(10):1938. doi: 10.3390/polym14101938.
4
Chemical composition, antioxidant activities and antibacterial activities of essential oil from Thunb. growing in South Africa.生长于南非的海州常山的精油的化学成分、抗氧化活性及抗菌活性
Heliyon. 2021 Jun 7;7(6):e07244. doi: 10.1016/j.heliyon.2021.e07244. eCollection 2021 Jun.
5
Chemical and Bioactivity Evaluation of and Polyphenolic-Rich Extracts.和多酚类物质丰富的提取物的化学和生物活性评价。
Biomed Res Int. 2019 Mar 12;2019:3692605. doi: 10.1155/2019/3692605. eCollection 2019.
6
Scientific Study of Gentiana kurroo Royle.匙叶龙胆的科学研究。
Medicines (Basel). 2017 Oct 12;4(4):74. doi: 10.3390/medicines4040074.
7
Potential antioxidant and lipid peroxidation inhibition of leaf extract in minced pork.猪肉糜中叶提取物潜在的抗氧化及脂质过氧化抑制作用
Asian-Australas J Anim Sci. 2017 Sep;30(9):1323-1331. doi: 10.5713/ajas.17.0036. Epub 2017 Apr 19.
8
Potential biological efficacy of Pinus plant species against oxidative, inflammatory and microbial disorders.松树植物物种对氧化、炎症和微生物紊乱的潜在生物学功效。
BMC Complement Altern Med. 2016 Jan 28;16:35. doi: 10.1186/s12906-016-1011-6.
9
Total phenolic and flavonoid content and antibacterial activity of Punica granatum L. var. pleniflora flowers (Golnar) against bacterial strains causing foodborne diseases.石榴（重瓣石榴品种）花（戈尔纳尔）对引起食源性疾病的细菌菌株的总酚和黄酮含量及抗菌活性。
BMC Complement Altern Med. 2015 Oct 15;15:366. doi: 10.1186/s12906-015-0887-x.
本文引用的文献
1
Induction of apoptosis in human pancreatic MiaPaCa-2 cells through the loss of mitochondrial membrane potential (ΔΨm) by Gentiana kurroo root extract and LC-ESI-MS analysis of its principal constituents.通过秦艽根提取物导致人胰腺 MiaPaCa-2 细胞线粒体膜电位（ΔΨm）丧失诱导细胞凋亡及其主要成分的 LC-ESI-MS 分析
Phytomedicine. 2013 Jun 15;20(8-9):723-33. doi: 10.1016/j.phymed.2013.01.011. Epub 2013 Mar 1.
2
Flavonoids as antioxidants in plants: location and functional significance.类黄酮作为植物中的抗氧化剂：位置和功能意义。
Plant Sci. 2012 Nov;196:67-76. doi: 10.1016/j.plantsci.2012.07.014. Epub 2012 Aug 11.
3
Chronic inflammation and mutagenesis.慢性炎症与突变。
Mutat Res. 2010 Aug 7;690(1-2):3-11. doi: 10.1016/j.mrfmmm.2010.03.007. Epub 2010 Mar 17.
4
Radical scavenging ability of polyphenolic compounds towards DPPH free radical.多酚类化合物对DPPH自由基的自由基清除能力。
Talanta. 2007 Jan 15;71(1):230-5. doi: 10.1016/j.talanta.2006.03.050. Epub 2006 May 2.
5
Antioxidant and antimicrobial activities of native Rosa sp. from British Columbia, Canada.加拿大不列颠哥伦比亚省原生蔷薇属植物的抗氧化和抗菌活性。
Int J Food Sci Nutr. 2007 May;58(3):178-89. doi: 10.1080/09637480601121318.
6
Oxidative stress and cancer: have we moved forward?氧化应激与癌症：我们取得进展了吗？
Biochem J. 2007 Jan 1;401(1):1-11. doi: 10.1042/BJ20061131.
7
Oxidative stress and neurodegeneration: where are we now?氧化应激与神经退行性变：我们目前处于什么阶段？
J Neurochem. 2006 Jun;97(6):1634-58. doi: 10.1111/j.1471-4159.2006.03907.x.
8
Antimicrobial activity of flavonoids.黄酮类化合物的抗菌活性。
Int J Antimicrob Agents. 2005 Nov;26(5):343-56. doi: 10.1016/j.ijantimicag.2005.09.002.
9
Purification and partial characterization of a low temperature responsive Mn-SOD from tea (Camellia sinensis (L.) O. Kuntze).茶叶（茶树（L.）O. 昆茨）中一种低温响应型锰超氧化物歧化酶的纯化及部分特性分析
Biochem Biophys Res Commun. 2005 Apr 15;329(3):831-8. doi: 10.1016/j.bbrc.2005.02.051.
10
Polyphenols: chemistry, dietary sources, metabolism, and nutritional significance.多酚类物质：化学性质、膳食来源、代谢及营养意义
Nutr Rev. 1998 Nov;56(11):317-33. doi: 10.1111/j.1753-4887.1998.tb01670.x.
Zotero 插件