National Institute for Research-Development of Biological Sciences, Bucharest 6, 060031, Romania.
Faculty of Applied Chemistry and Materials Science, Politehnica University of Bucharest, Bucharest, Romania.
Biomed Res Int. 2019 Mar 12;2019:3692605. doi: 10.1155/2019/3692605. eCollection 2019.
This study evaluated the biological activities of and extracts enriched in polyphenols obtained by nanofiltration. The HPLC-MS analysis showed that contains mainly flavonoids, especially rutin, while in extracts show the high concentration of the phenolic acids, principally the chlorogenic acid and sinapic acid. Herein, there is the first report of ursolic acid, genistin, and isorhamnetin in and polyphenolic-rich extract showed high scavenging activity (IC=0.0081 mg/mL) comparable to that of standard compound (ascorbic acid) and a higher reducing power (IC= 0.082 mg/mL), with IC having a significantly lower value than IC for ascorbic acid. Both extracts were nontoxic to NCTC cell line. Among the investigated herbs, polyphenolic-rich extract showed the highest inhibitory activities with the IC value of 31.3 g/mL for lipoxygenase and 24.6 g/mL for hyaluronidase. Both polyphenolic-rich extracts had a higher inhibitory effect on -amylase and -glucosidase than that of the acarbose. The synergistic effect of ursolic acid, rutin, chlorogenic acid, rosmarinic acid, genistin, and daidzein identified in polyphenolic-rich extracts could be mainly responsible for the pharmacological potentials of the studied extracts used in managing inflammation and diabetes.
本研究评估了纳滤法富集多酚的 和 提取物的生物活性。HPLC-MS 分析表明， 中主要含有类黄酮，特别是芦丁，而 提取物中则显示出高浓度的酚酸，主要是绿原酸和芥子酸。本文首次报道了熊果酸、染料木素和异鼠李素存在于 和 多酚丰富的提取物中，其具有高的清除活性（IC=0.0081 mg/mL），可与标准化合物（抗坏血酸）相媲美，且具有更高的还原能力（IC=0.082 mg/mL），IC 值明显低于抗坏血酸的 IC 值。两种提取物对 NCTC 细胞系均无毒性。在所研究的草药中， 多酚丰富的提取物对脂氧合酶的 IC 值为 31.3 g/mL，对透明质酸酶的 IC 值为 24.6 g/mL，表现出最高的抑制活性。两种多酚丰富的提取物对 -淀粉酶和 -葡萄糖苷酶的抑制作用均高于阿卡波糖。多酚丰富的提取物中鉴定出的熊果酸、芦丁、绿原酸、迷迭香酸、染料木素和大豆苷元的协同作用可能是这些提取物用于管理炎症和糖尿病的药理潜力的主要原因。
