Aghabozorg Afjeh Sarah Sadat, Ghaderian Sayyed Mohammad Hossein, Mirfakhraie Reza, Piryaei Mohammad, Zaim Kohan Hooshang
Department of Medical Genetics, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Department of Medical Genetics, Tehran University of Medical Sciences, Tehran, Iran.
Int J Mol Cell Med. 2014 Summer;3(3):157-65.
Cardiovascular disease has become the main factor of death and birth defects in the world and also in Iran. New clinical studies have shown that early diagnosis of patients with coronary artery disease (CAD) can contribute to effective prevention or therapeutic structures, which reduce mortality or the next chance of cardiovascular events, and increase the quality of life. Most studies on CAD disease and its genetic risk factors so far, have been done excluding the Iranian population. PubMed was used to search for all relevant studies published on or before 2013 and rs3184504 was selected for association study for CAD. A total of 200 subjects with 100 cases and 100 controls were ultimately included in the analysis. Blood samples were collected and after DNA extraction the DNA analysis was performed by TaqMan Probe Real Time PCR to evaluate the association between candidate variant with the disease and some blood biochemical factors. Our study demonstrated that there was not a direct association between rs3184504 C>T variant with risk of CAD in Iranian population, whereas, there is a significant association between this variant with increased blood LDL and diastolic blood pressure. Further molecular analysis and other disease association studies are necessary in the Iranian population.
心血管疾病已成为全球以及伊朗死亡和出生缺陷的主要因素。新的临床研究表明，对冠心病（CAD）患者进行早期诊断有助于建立有效的预防或治疗体系，从而降低死亡率或减少心血管事件再次发生的几率，并提高生活质量。迄今为止，大多数关于CAD疾病及其遗传风险因素的研究都未将伊朗人群纳入。利用PubMed搜索2013年及以前发表的所有相关研究，并选择rs3184504进行CAD关联研究。最终共有200名受试者纳入分析，其中100例病例和100例对照。采集血样，提取DNA后，采用TaqMan探针实时荧光定量PCR进行DNA分析，以评估候选变异与疾病及一些血液生化因素之间的关联。我们的研究表明，在伊朗人群中，rs3184504 C>T变异与CAD风险之间不存在直接关联，然而，该变异与血液低密度脂蛋白（LDL）升高和舒张压之间存在显著关联。在伊朗人群中，有必要进行进一步的分子分析和其他疾病关联研究。
