1 State Key Laboratory for Diagnosis and Treatment of Infectious Disease, The First Affiliated Hospital, School of Medicine, Zhejiang University , Hangzhou, People's Republic of China .
DNA Cell Biol. 2015 Jan;34(1):29-36. doi: 10.1089/dna.2014.2522.
microRNAs are endogenous noncoding RNA molecules of ∼22 nucleotides that regulate gene function by modification of target mRNAs. Due to tissue specific of miR-133a and miR-1/206 for skeletal muscles, we investigated the role of miR-133a and miR-1/206 in promoting the differentiation of the C2C12 cells. The results show that directly transfecting mature miR-133a, miR-1/206, or combinations (miR-1 and miR-206, miR-1 and miR-133a, and miR-133a and miR-206) into C2C12 cells, respectively, for 5 days induces formation of myogenic progenitor cells. Overexpression of miR-133a and miR-206 in C2C12 cells greatly improved multinucleated myotube formation. microRNA-133a (miR-133a) is highly expressed during human muscle development. Using bioinformatics, we identified one putative miR-133a binding site within the 3'-untranslated region of the mouse Foxl2 mRNA. The expression of Foxl2 was shown to be downregulated by subsequent western blot analysis.
microRNAs 是内源性的非编码 RNA 分子，大小约为 22 个核苷酸，通过靶 mRNA 的修饰来调节基因功能。由于 miR-133a 和 miR-1/206 对骨骼肌具有组织特异性，我们研究了 miR-133a 和 miR-1/206 在促进 C2C12 细胞分化中的作用。结果表明，直接将成熟的 miR-133a、miR-1/206 或组合（miR-1 和 miR-206、miR-1 和 miR-133a、miR-133a 和 miR-206）转染到 C2C12 细胞中 5 天，分别诱导肌原细胞的形成。在 C2C12 细胞中转染 miR-133a 和 miR-206 可显著提高多核肌管的形成。miR-133a 在人类肌肉发育过程中高度表达。通过生物信息学，我们在 Foxl2 mRNA 的 3'非翻译区发现了一个潜在的 miR-133a 结合位点。随后的 Western blot 分析显示 Foxl2 的表达被下调。
