利用血液和尿液的荧光发射光谱和同步荧光激发光谱检测肾癌的研究。

A study for the detection of kidney cancer using fluorescence emission spectra and synchronous fluorescence excitation spectra of blood and urine.

机构信息

Physics and Astronomy Department, College of Science, King Saud University, Riyadh, Saudi Arabia; Research Chair in Laser Diagnosis of Cancer, Department of Physics and Astronomy, College of Science, King Saud University, Saudi Arabia.

出版信息

Photodiagnosis Photodyn Ther. 2018 Sep;23:40-44. doi: 10.1016/j.pdpdt.2018.05.012. Epub 2018 May 22.

DOI:10.1016/j.pdpdt.2018.05.012

PMID:29800712

Abstract

In this study, we compared different types of biomolecular markers in kidney cancer patients and in normal healthy controls, using fluorescence emission spectra and synchronous fluorescence excitation spectra. We were able to provide an accurate classification of the spectral features of kidney cancer patients relative to that of normal controls, in terms of the concentration ratios of biomolecules (viz., tryptophan, NADH, FAD, basic porphyrin, and acidic porphyrin) based on the intensity of their spectral peaks. The specificity and sensitivity of the method were 90%. The rationale of our current approach is to evolve an innovative protocol for the spectral characterization of in vitro optical analyses suitable for both small clinics and hospitals.

摘要

在这项研究中，我们使用荧光发射光谱和同步荧光激发光谱比较了肾癌患者和正常健康对照者的不同类型生物分子标志物。我们能够根据生物分子（色氨酸、NADH、FAD、碱性卟啉和酸性卟啉）浓度比的光谱峰强度，提供对肾癌患者和正常对照者光谱特征的准确分类。该方法的特异性和灵敏度为 90%。我们当前方法的基本原理是为适合小型诊所和医院的体外光学分析的光谱特征制定一个创新的方案。